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Cell and Tissue Research

, Volume 372, Issue 3, pp 535–547 | Cite as

Induction of human umbilical cord mesenchymal stem cells into tissue-forming cells in a murine model: implications for pelvic floor reconstruction

  • Jing Ding
  • Qin Han
  • Mou Deng
  • Xiao-chen Song
  • Chun Chen
  • Fang-fang Ai
  • Lan Zhu
  • Robert Chun-hua Zhao
Regular Article

Abstract

HUMSCs were isolated, differentiated and characterized in vitro. Both HUMSCs and smooth muscle cells differentiated from HUMSCs were used to fabricate tissue-engineered fascia equivalents. Forty-eight mature female Sprague Dawley rats were randomly assigned to four groups: group A (GynemeshTMPS, n = 12), group B (GynemeshTMPS + HUMSCs; n = 12), group C (GynemeshTMPS + smooth muscle cells differentiated from HUMSCs; n = 12) and group D (GynemeshTMPS + HUMSCs + smooth muscle cells differentiated from HUMSCs; n = 12). The posterior vaginal wall was incised from the introitus and the mesh was then implanted. Three implants of each type were tested at 1, 4, 8 and 12 weeks. Fibrotic remodeling, inflammation, vascularization and tissue regeneration were histologically assessed. The levels of type I and type III collagen were determined. There was no difference in fibrotic remodeling between cell-seeded and unseeded meshes at any time (p > 0.05). At 12 weeks, there did not appear to be fewer inflammatory cells around the filament bundles in the mesh with cells compared with the mesh alone (P > 0.05). Group D showed a trend toward better vascularization at 12 weeks compared with group A (P < 0.05). Twelve weeks after implantation, a thin layer of new tissue growth covered the unseeded scaffold and a thicker layer covered the cell-seeded scaffold (P < 0.05). No significant difference in the ratio of collagen type I/III could be detected among the different groups after 12 weeks (P > 0.05). HUMSCs with differentiated smooth muscle cells might have a potential role in fascia tissue engineering to repair POP in the future.

Keywords

Human umbilical cord mesenchymal stem cells Smooth muscle cells Polypropylene Tissue engineering Pelvic floor reconstruction 

Notes

Acknowledgements

We thank the animal care staff at PUMCH for their assistance in managing the husbandry of the rats used in this study. We are grateful to Mrs. Xiao-yan Cheng for her excellent assistance during this experiment.

Authors’ contributions

J Ding: Protocol development, data collection, data analysis and manuscript writing.

Q Han: Protocol development, data collection, data analysis and manuscript writing.

M Deng: Analysis of tissue response in the animal model.

X-C Song: Data collection.

C Chen: Manuscript writing.

F-f Ai: Data analysis.

L Zhu: Protocol development.

R Chun-hua Zhao: Protocol development.

Funding

This study received financial support from the National Natural Science Foundation of China (No. 81571421) and the Postdoctoral Foundation of China (2016 M600263).

Compliance with ethical standards

Conflict of interest

None.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jing Ding
    • 1
    • 2
  • Qin Han
    • 3
  • Mou Deng
    • 1
  • Xiao-chen Song
    • 1
  • Chun Chen
    • 1
  • Fang-fang Ai
    • 1
  • Lan Zhu
    • 1
    • 4
  • Robert Chun-hua Zhao
    • 3
    • 4
    • 5
  1. 1.Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina
  2. 2.Department of GynecologyHarbin Medical University Cancer HospitalHarbinChina
  3. 3.Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical CollegeTissue Engineering Center of Chinese Academy of Medical SciencesBeijingChina
  4. 4.BeijingChina
  5. 5.Center of Translational Medicine, Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

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