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Mechanism of Lycium barbarum polysaccharides on primary cultured rat hippocampal neurons

Abstract

Lycium barbarum polysaccharides (LBP) have been reported to have a wide range of beneficial effects including neuroprotection, anti-aging and anticancer. However, the anti-inflammation mechanism of LBP on primary cultured rat hippocampal neurons injured by oxygen-glucose deprivation/reperfusion (OGD/RP) is incompletely understood. We investigate the neuroprotective effects of LBP on neonatal rat primary cultured hippocampal neurons injured by OGD/RP with different approaches: MTT assay was used to detect cell viability, lactate dehydrogenase leakage was used to detect neuronal damage, formation of reactive oxygen species was determined by using fluorescent probe DCFH-DA. Hoechst 33,342 staining and TUNEL staining were used to determine the cell apoptosis. JC-1 was used to evaluate loss of mitochondrial membrane potential (MMP). The fluorescence intensity of [Ca2+]i in hippocampal neurons was determined by laser scanning confocal microscopy. The expression of various apoptotic markers such as TLR4, IκB, IL-6 and NF-κB were investigated by RT-PCR and western blot analysis. Results from each approach demonstrated that LBP increased the cell abilities and decreased the cell morphologic impairment. Furthermore, LBP increased MMP but inhibited [Ca2+]i elevation and significantly suppressed overexpression of NF-κB, IL-6 TLR4 and increased IκB expression.

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Acknowledgements

The authors gratefully acknowledge the financial support by the National Natural Science Foundation of China (Grant no 81360649), the Natural Science Foundation of Ningxia (Grant no. NZ13278) and Ningxia Hui Autonomous Region, colleges and universities of science and technology research projects (grant no. XM2011017).

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Correspondence to Jian-Qiang Yu.

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Zhao, P., Ma, N., Chang, R. et al. Mechanism of Lycium barbarum polysaccharides on primary cultured rat hippocampal neurons. Cell Tissue Res 369, 455–465 (2017). https://doi.org/10.1007/s00441-017-2648-2

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Keywords

  • Oxygen-glucose deprivation/reperfusion
  • Lycium barbarum polysaccharide
  • Toll-like receptor 4
  • Nuclear factor kappa B
  • Inflammatory cytokines