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Activating transcription factor 5 (ATF5) is essential for the maturation and survival of mouse basal vomeronasal sensory neurons

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Abstract

Activating transcription factor 5 (ATF5) is a member of the CREB/ATF family of transcription factors, which is highly expressed in olfactory chemosensory tissues, the main olfactory epithelium and vomeronasal epithelium (VNE) in mice. The vomeronasal sensory neurons in the VNE detect pheromones in order to regulate social behaviors such as mating and aggression; however, the physiological role of ATF5 in the vomeronasal sensory system remains unknown. In this study, we found that the differentiation of mature vomeronasal sensory neurons, assessed by olfactory marker protein expression, was inhibited in ATF5-deficient VNE. In addition, many apoptotic vomeronasal sensory neurons were evident in ATF5-deficient VNE. The vomeronasal sensory neurons consist of two major types of neuron expressing either vomeronasal 1 receptor (V1r)/Gαi2 or vomeronasal 2 receptor (V2r)/Gαo. We demonstrated that the differentiation, survival and axonal projection of V2r/Gαo-type rather than V1r/Gαi2-type vomeronasal sensory neurons were severely inhibited in ATF5-deficient VNE. These results suggest that ATF5 is one of the transcription factors crucial for the vomeronasal sensory formation.

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Acknowledgment

This work was supported by a grant-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Author information

Correspondence to Yuji Takahashi.

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Suppl Fig. S1
figure7

Double immunofluorescence with anti-OMP and anti- Gαi2 for AOB imaging analysis. Double immunofluorescence of OMP (a, b) and Gαi2 (c, d) with the merge image (e, f) on adult ATF5+/+ and ATF5-/- accessory olfactory bulb (AOB). A and P indicate anterior and posterior direction, respectively. The dotted line delineates the region of vomeronasal nerve layer and glomerular layer (vnl-gl) of the AOB. Arrowheads indicate the border between the anterior and posterior region of the AOB. Scale bars 100 μm. (GIF 27 kb)

High resolution image (TIFF 4883 kb)

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Nakano, H., Iida, Y., Suzuki, M. et al. Activating transcription factor 5 (ATF5) is essential for the maturation and survival of mouse basal vomeronasal sensory neurons. Cell Tissue Res 363, 621–633 (2016). https://doi.org/10.1007/s00441-015-2283-8

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Keywords

  • Activating transcription factor
  • Vomeronasal sensory neuron
  • Apoptosis
  • Pheromone
  • Mouse