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Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes

Abstract

Endonuclease-induced DNA fragmentation is a hallmark of apoptosis. DNase gamma (DNase γ) was recently identified as one of the endonucleases responsible for apoptotic DNA fragmentation. In this study, immunohistochemistry for DNase γ was performed on paraffin sections of rodent liver in well-defined models of hepatocyte apoptosis induced by Fas antibody (Fas) or cycloheximide (CHX), and necrosis induced by lipopolysaccharide (LPS) or carbon tetrachloride (CCl4). DNase γ immunoreactivity was compared with TdT-mediated dUTP nick-end labeling (TUNEL) reactivity. Our results showed TUNEL reactivity in both apoptotic and necrotic hepatocytes. DNase γ immunoreactivity was not detected during LPS-induced or CCl4-induced hepatocyte necrosis. In contrast, it was evident during CHX-induced, but not Fas-induced, apoptotic DNA fragmentation. These findings suggest that DNase γ plays an important role in Fas-independent apoptotic DNA fragmentation in hepatocytes.

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Correspondence to Yoshikazu Higami.

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Higami, Y., Tsuchiya, T., To, K. et al. Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes. Cell Tissue Res 316, 403–407 (2004). https://doi.org/10.1007/s00441-004-0890-x

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Keywords

  • DNase gamma
  • Hepatocytes
  • Apoptosis
  • Fas
  • Cycloheximide
  • Rat (F344)
  • Mouse (BALB/c)