Guanidinium chloride treatment of Sepia officinalis cartilage solubilized a component that contained hydroxyproline. Electron-microscopy observation of rotary-shadowed preparations of this component revealed it to consist of rod-like units themselves consisting of filaments. Dialysis of an acetic acid solution against ATP afforded polymeric aggregates consisting of a succession of two or three thick sections showing transverse electron-opaque banding, separated by thinner sections without banding. Electrophoresis produced a main band of about 140 kDa sensitive to bacterial collagenase. After reduction with mercaptoethanol, electrophoresis afforded a 40-kDa band. Pepsin digestion resulted in additional electrophoretic bands. These data suggest the presence of a collagen in Sepia cartilage with characteristics unlike those of any known collagen.
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Rigo, C., Bairati, A. A new collagen from the extracellular matrix of Sepia officinalis cartilage. Cell Tissue Res 310, 253–256 (2002). https://doi.org/10.1007/s00441-002-0619-7
- Cartilage Collagen Electron microscopy Electrophoresis Sepia officinalis (Mollusca, Cephalopoda)