Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

A new collagen from the extracellular matrix of Sepia officinalis cartilage

  • 39 Accesses

  • 4 Citations


Guanidinium chloride treatment of Sepia officinalis cartilage solubilized a component that contained hydroxyproline. Electron-microscopy observation of rotary-shadowed preparations of this component revealed it to consist of rod-like units themselves consisting of filaments. Dialysis of an acetic acid solution against ATP afforded polymeric aggregates consisting of a succession of two or three thick sections showing transverse electron-opaque banding, separated by thinner sections without banding. Electrophoresis produced a main band of about 140 kDa sensitive to bacterial collagenase. After reduction with mercaptoethanol, electrophoresis afforded a 40-kDa band. Pepsin digestion resulted in additional electrophoretic bands. These data suggest the presence of a collagen in Sepia cartilage with characteristics unlike those of any known collagen.

This is a preview of subscription content, log in to check access.

Author information

Additional information

Electronic Publication

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Rigo, C., Bairati, A. A new collagen from the extracellular matrix of Sepia officinalis cartilage. Cell Tissue Res 310, 253–256 (2002). https://doi.org/10.1007/s00441-002-0619-7

Download citation

  • Cartilage Collagen Electron microscopy Electrophoresis Sepia officinalis (Mollusca, Cephalopoda)