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Regulation of the yeast HIS7 gene by the global transcription factor Abf1p


The HIS7 gene of Saccharomyces cerevisiae encodes a bifunctional glutamine amidotransferase: cyclase that catalyzes the formation of biosynthetic precursors for histidine and adenine. HIS7 is activated by Gcn4p upon amino acid starvation and by the Bas1/2p complex in response to adenine limitation. Mutation analysis of the HIS7 promoter in a Δgcn4 background revealed a polyd(A/T) stretch and a d(CT) repeat as essential elements for Gcn4p-independent basal HIS7 transcription. The protein binding this element was enriched and identified as the multifunctional DNA-binding protein Abf1p. Abf1p binds specifically to the d(CT) repeat sequence, which represents a novel Abf1p-binding motif, and protects 17 nucleotides from digestion by DNase I. In addition, Abf1p binding causes bending of the HIS7 promoter structure.

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Received: 22 November 1996 / Accepted: 26 February 1997

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Springer, C., Krappmann, S., Künzler, M. et al. Regulation of the yeast HIS7 gene by the global transcription factor Abf1p. Mol Gen Genet 256, 136–146 (1997).

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  • Key wordsSaccharomyces cerevisiae
  • Regulation of transcription
  • Histidine biosynthesis
  • HIS7
  • Abf1p