PCTAIRE-1 is a member of the cyclin-dependent kinase (cdk)-like class of proteins, and is localized mainly in the mammalian brain. Using the yeast two-hybrid system we screened a mouse brain cDNA library with PCTAIRE-1 as bait, and isolated several clones coding for the mouse homologs of the following proteins: p11 (also known as calpactin I light chain) and the η, θ (also known asτ) and ζ isoforms of 14-3-3 proteins. We confirmed that these four proteins interact with PCTAIRE-1 by demonstrating the biochemical interactions using the pure recombinant proteins. The fact that 14-3-3 proteins are known to interact with many other intracellular proteins (such as C-kinase, Raf, Bcr, PI3-kinase) and p11 with annexin II (a major pp60v-src and C-kinase substrate) suggests that PCTAIRE-1 might be part of multiple signal transduction cascades and cellular protein networks.
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Received: 23 September 1996 / Accepted: 10 January 1997
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Sladeczek, F., Camonis, J., Burnol, A. et al. The Cdk-like protein PCTAIRE-1 from mouse brain associates with p11 and 14-3-3 proteins. Mol Gen Genet 254, 571–577 (1997). https://doi.org/10.1007/s004380050453
- Key words Cdk-like protein PCTAIRE-1
- Two-hybrid system
- Protein-protein interactions
- Annexin II-associated protein p11
- 14-3-3 proteins