The organization of the actin cytoskeleton plays an integral role in cell morphogenesis of all eukaryotes. We have isolated a temperature-sensitive mutant in Schizosaccharomyces pombe, wat1-1, in which acting patches are delocalized, resulting in an elliptically shaped cell phenotype. Molecular cloning and DNA sequencing of wat1 + showed that the gene encodes a 314 residue protein containing WD-40 repeats. Cells lacking wat1 + are slow growing but viable at 25° C and temperature-sensitive for growth above 33° C. At restrictive temperature, wat1-d strains are phenotypically indistinguishable from wat1-1. When combined with a deletion for the wat1 + gene, cdc mutants failed to elongate at restrictive temperature and exhibited alterations in actin patch localization. This analysis suggests that wat1 + is required directly or indirectly for polarized cell growth in S. pombe. Wat1p and a functional, epitope-tagged, version of Wat1p can be overproduced without inducing alterations in cell morphology.
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Received: 18 September 1996 / Accepted: 22 October 1996
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Kemp, J., Balasubramanian, M. & Gould, K. A wat1 mutant of fission yeast is defective in cell morphology. Mol Gen Genet 254, 127–138 (1997). https://doi.org/10.1007/s004380050400
- Key words Fission yeast
- WD-40 repeats
- Cell polarity