Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Complementation of a yeast top2 ts mutation by a cDNA encoding rat DNA topoisomerase IIα

  • 40 Accesses

  • 1 Citations


 A series of yeast expression plasmids which comprise segments of the cDNA sequences encoding rat topo IIα have been constructed. The transcription of these constructs is under the control of the yeast GAL1 promoter. Galactose-dependent expression of the cloned rat topo IIα cDNA complemented a yeast top2 ts mutation, as well as a deletion mutation at the yeast TOP2 locus. Truncation of 12 N-terminal amino acids and/or 158 C-terminal amino acids of rat topo IIα had no effect on its ability functionally to substitute for top2ts. Moreover, a cDNA construct with mutated putative leucine zipper domain (amino acids 993–1013) retained the complementation activity. These observations suggest that transformants capable of conditional topo IIα expression can be exploited as a useful model system for studies on the structure-function relationships of wild-type and mutated topo IIα, as well as the interplay of potential antitumor drugs with the enzyme.

This is a preview of subscription content, log in to check access.

Author information

Additional information

Received: 4 April 1996/Accepted: 15 July 1996

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Yoon, J., Park, S., Cho, H. et al. Complementation of a yeast top2 ts mutation by a cDNA encoding rat DNA topoisomerase IIα. Mol Gen Genet 253, 81–88 (1996). https://doi.org/10.1007/s004380050299

Download citation

  • Key words Rat
  • DNA topoisomerase IIα
  • Saccharomyces cerevisiae
  • top2ts
  • Complementation
  • Leucine zipper