Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The ITR binding domain of the Mariner Mos-1 transposase

Abstract

Mariner-like elements are widespread eukaryotic transposons, but Mos-1 is the only natural element that is known to be active. Little is known about the biochemistry of mariner transposition. The first step in the process is the binding of the transposase to the 5′ and 3′ inverted terminal repeats (ITRs) of the element. Using the 3′ ITR of the element, we have determined the binding properties of a recombinant Mos-1 transposase produced in bacteria, and we have used deletion derivatives to localize the minimal ITR binding domain between amino acids 1 and 141. Its features and structure indicate that it differs from the ITR binding domain of the transposase encoded by Tc1-related elements.

This is a preview of subscription content, log in to check access.

Author information

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Augé-Gouillou, C., Hamelin, M., Demattei, M. et al. The ITR binding domain of the Mariner Mos-1 transposase. Mol Gen Genomics 265, 58–65 (2001). https://doi.org/10.1007/s004380000386

Download citation

  • Mariner Transposase DNA-binding properties Transposition