We have determined the distribution of Y-chromosomal haplotypes and haplogroups in the Yong population, one of the largest and well-known ethnic groups that began migrating southward from China to Thailand centuries ago. Their unique mass migration pattern provided great opportunities for researchers to study the genetic links of the transboundary migration movements among the peoples of China, Myanmar and Thailand. We analysed relevant male-specific markers, such as Y-STRs and Y-SNPs, and the distribution of 23 Y-STRs of 111 Yong individuals and 116 nearby ethnic groups including the Shan, Northern Thai, Lawa, Lua, Skaw, Pwo and Padong groups. We found that the general haplogroup distribution values were similar among different populations; however, the haplogroups O1b-M268 and O2-M112 constituted the vast majority of these values. In contrast with previous maternal lineage studies, the paternal lineage of the Yong did not relate to the Xishuangbanna Dai people, who represent their historically documented ancestors. However, they did display a close genetic affinity to other prehistoric Tai-Kadai speaking groups in China such as the Zhuang and Bouyei. Low degrees of genetic admixture within the populations who belonged to the Austroasiatic and Sino-Tibetan linguistic families were observed in the gene pool of the Yong populations. Resettlement in northern Thailand in the early part of the nineteenth century AD, by way of mass migration trend, was able to preserve the Yong’s ancestral genetic background in terms of the way they had previously lived in China and Myanmar. Our study has revealed similar genetic structures among ethnic populations in northern Thailand and southern China, and has identified and emphasized an ancient Tai-Kadai patrilineal ancestry line in the Yong ethnic group.
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Bai H, Guo X, Zhang D, Narisu N, Bu J, Jirimutu J, Liang F, Zhao X, Xing Y, Wang D, Li T, Zhang Y, Guan B, Yang X, Yang Z, Shuangshan S, Su Z, Wu H, Li W, Chen M, Zhu S, Bayinnamula B, Chang Y, Gao Y, Lan T, Suyalatu S, Huang H, Su Y, Chen Y, Li W, Yang X, Feng Q, Wang J, Yang H, Wang J, Wu Q, Yin Y, Zhou H (2014) The genome of a Mongolian individual reveals the genetic imprints of Mongolians on modern human populations. Genome Biol Evol 6(12):3122–3136
Balanovsky O, Zhabagin M, Agdzhoyan A, Chukhryaeva M, Zaporozhchenko V, Utevska O, Highnam G, Sabitov Z, Greenspan E, Dibirova K, Skhalyakho R, Kuznetsova M, Koshel S, Yusupov Y, Nymadawa P, Zhumadilov Z, Pocheshkhova E, Haber M, Zalloua P, Yepiskoposyan L, Dybo A, Tyler-Smith C, Balanovska E (2015) Deep phylogenetic analysis of haplogroup G1 provides estimates of SNP and STR mutation rates on the human Y-chromosome and reveals migrations of Iranic speakers. PLoS ONE 10(4):e0122968
Bandelt H-J, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:37–48
Bellwood P (2011) The checkered prehistory of rice movement southwards as a domesticated cereal-from the Yangzi to the equator. Rice 4:93–103
Besaggio D, Fuselli S, Srikummool M, Kampuansai J, Castrì L, Tyler-Smith C, Seielstad M, Kangwanpong D, Bertorelle G (2007) Genetic variation in Northern Thailand Hill Tribes: origins and relationships with social structure and linguistic differences. BMC Evol Biol 7(2):s12
Bíró AZ, Zalán A, Völgyi A, Pamjav H (2009) A Y-chromosomal comparison of the Madjars (Kazakhstan) and the Magyars (Hungary). Am J Phys Anthropol 139(3):305–310
Bíró A, Fehér T, Bárány G, Pamjav H (2015) Testing Central and Inner Asian admixture among contemporary Hungarians. Forensic Sci Int Genet 15:121–126
Brunelli A, Kampuansai J, Seielstad M, Lomthaisong K, Kangwanpong D, Ghirotto S, Kutanan W (2017) Y chromosomal evidence on the origin of northern Thai people. PLoS ONE 12(7):e0181935
Chaoxiong Z (2005) Study of the origin in Zhuang civilization. Guangxi People’s Publishing House, Naning
Excoffier L, Lischer L (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Eco Res 10:564–567
Fuller DQ (2011) Pathways to Asian civilizations: tracing the origins and spread of rice and rice cultures. Rice 4:78–92
Hammer MF, Karafet TM, Park H, Omoto K, Harihara S, Stoneking M, Horai S (2006) Dual origins of the Japanese: common ground for hunter-gatherer and farmer Y chromosomes. J Hum Genet 51:47–58
Ilumäe AM, Reidla M, Chukhryaeva M, Järve M, Post H, Karmin M, Saag L, Agdzhoyan A, Kushniarevich A, Litvinov S, Ekomasova N, Tambets K, Metspalu E, Khusainova R, Yunusbayev B, Khusnutdinova EK, Osipova LP, Fedorova S, Utevska O, Koshel S, Balanovska E, Behar DM, Balanovsky O, Kivisild T, Underhill PA, Villems R, Rootsi S (2016) Human Y chromosome haplogroup N: a non-trivial time-resolved phylogeography that cuts across language families. Am J Hum Genet 99:163–173
Kampuansai J, Kutanan W, Phuphanitcharoenkul S, Kangwanpong D (2012) A suggested Khmuic origin of the hunter-gatherer Mlabri in northern Thailand: evidence from maternal DNA lineages. Thai J Genet 5(2):203–215
Kampuansai J, Kutanan W, Tassi F, Kaewgahya M, Ghirotto S, Kangwanpong D (2016) Effect of migration patterns on maternal genetic structure: a case of Tai-Kadai migration from China to Thailand. J Hum Genet 62:223–228
Karafet T, Xu L, Du R, Wang W, Feng S, Wells RS, Redd AJ, Zegura SL, Hammer MF (2001) Paternal population history of East Asia: sources, patterns, and microevolutionary processes. Am J Hum Genet 69:615–628
Karafet TM, Mendez FL, Meilerman MB, Underhill PA, Zegura SL, Hammer MF (2008) New binary polymorphisms reshape and increase resolution of the human Y chromosomal haplogroup tree. Genom Res 18:830–838
Kim S, Kim K, Shin D, Jin H, Kwak K, Han M, Song J, Kim W, Kim W (2011) High frequencies of Y-chromosome haplogroup O2b-SRY465 lineages in Korea: a genetic perspective on the peopling of Korea. Investig Genet 2:10
Kutanan W, Kangwanpong D (2010) Genetic structure of the ethnic populations Lua and Htin in northern Thailand. Thai J Genet 3(2):160–171
Kutanan W, Kampuansai J, Fuselli S, Nakbunlung S, Seielstad M, Bertorelle G, Kangwanpong D (2011a) Genetic structure of the Mon-Khmer speaking groups and their affinity to the neighbouring Tai populations in Northern Thailand. BMC Genet 12:56
Kutanan W, Kampuansai J, Colonna V, Nakbunlung S, Lertvicha P, Seielstad M, Bertorelle G, Kangwanpong D (2011b) Genetic affinity and admixture of northern Thai people along their migration route in northern Thailand: evidence from autosomal STR loci. JHG 56(2):130–137
Lipson M, Cheronet O, Mallick S, Rohland N, Oxenham M, Pietrusewsky M, Pryce TO, Willis A, Matsumura H, Buckley H, Domett K, Nguyen GH, Trinh HH, Kyaw AA, Win TT, Pradier B, Broomandkhoshbacht N, Candilio F, Changmai P, Fernandes D, Ferry M, Gamarra B, Harney E, Kampuansai J, Kutanan W, Michel M, Novak M, Oppenheimer J, Sirak K, Stewardson K, Zhang Z, Flegontov P, Pinhasi R, Reich D (2018) Ancient genomes document multiple waves of migration in Southeast Asian prehistory. Science 361(6397):92–95
Lithanatudom P, Wipasa J, Inti P, Chawansuntati K, Svasti S, Fucharoen S, Kangwanpong D, Kampuansai J (2016) Hemoglobin E prevalence among ethnic groups residing in malaria-endemic areas of northern Thailand and its Lack of association with Plasmodium falciparum invasion in vitro. PLoS ONE 11(1):e0148079
Malasam S (2001) On the migration and settlement of the Yong people in Lamphun, Thailand from 1805-1902. Thammasat University Press, Bangkok
Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323
Ongsakul S (2005) History of Lanna. Silkworm Books, Chiang Mai
Pamjav H, Fóthi Á, Fehér T, Fóthi E (2017) A study of the Bodrogköz population in north-eastern Hungary by Y chromosomal haplotypes and haplogroups. Mol Genet Genomics 292(4):883–894
Penth H, Forbes A (2004) A brief history of Lanna and the peoples of Chiang Mai. O.S. Printing House, Bangkok
Poznik GD, Xue Y, Mendez FL, Willems TF, Massaia A, Wilson Sayres MA, Ayub Q, McCarthy SA, Narechania A, Kashin S, Chen Y, Banerjee R, Rodriguez-Flores JL, Cerezo M, Shao H, Gymrek M, Malhotra A, Louzada S, Desalle R, Ritchie GR, Cerveira E, Fitzgerald TW, Garrison E, Marcketta A, Mittelman D, Romanovitch M, Zhang C, Zheng-Bradley X, Abecasis GR, McCarroll SA, Flicek P, Underhill PA, Coin L, Zerbino DR, Yang F, Lee C, Clarke L, Auton A, Erlich Y, Handsaker RE, 1000 Genomes Project Consortium, Bustamante CD, Tyler-Smith C (2016) Punctuated bursts in human male demography inferred from 1,244 worldwide Y-chromosome sequences. Nat Genet 48(6):593–599
Schliesinger J (2000) Ethnic groups of Thailand: Non-Tai-Speaking peoples. White Lotus Press, Bangkok
Schliesinger J (2001) Tai groups of Thailand: volume 1 introduction and overview. White Lotus Press, Bangkok
Seielstad M, Bekele E, Ibrahim M, Toure A, Traore M (1999) A view of modern human origins from Y chromosome microsatellite variation. Genome Res 9:558–567
Shi H, Zhong H, Peng Y et al (2008) Y chromosome evidence of earliest modern human settlement in East Asia and multiple origins of Tibetan and Japanese populations. BMC Biol 6:45
Underhill PA, Shen P, Lin AA, Jin L, Passarino G, Yang WH, Kauffman E, Bonné-Tamir B, Bertranpetit J, Francalacci P, Ibrahim M, Jenkins T, Kidd JR, Mehdi SQ, Seielstad MT, Wells RS, Piazza A, Davis RW, Feldman MW, Cavalli-Sforza LL, Oefner PJ (2000) Y chromosome sequence variation and the history of human populations. Nat Genet 26(3):358–361
Underhill PA, Myres NM, Rootsi S, Metspalu M, Zhivotovsky LA, King RJ, Lin AA, Chow CE, Semino O, Battaglia V, Kutuev I, Järve M, Chaubey G, Ayub Q, Mohyuddin A, Mehdi SQ, Sengupta S, Rogaev EI, Khusnutdinova EK, Pshenichnov A, Balanovsky O, Balanovska E, Jeran N, Augustin DH, Baldovic M, Herrera RJ, Thangaraj K, Singh V, Singh L, Majumder P, Rudan P, Primorac D, Villems R, Kivisild T (2010) Separating the post-Glacial coancestry of European and Asian Y chromosomes within haplogroup R1a. Eur J Hum Genet 18(4):479–484
Willuweit S, Roewer L (2015) The new Y chromosome haplotype reference database. Forensic Sci Int Genet 15:43–48
Yan S, Wang CC, Li H, Li SL, Jin L, The Genographic Consortium (2011) An updated tree of Y-chromosome Haplogroup O and revised phylogenetic positions of mutations P164 and PK4. Eur J Hum Genet 19:1013–1015
Zhang X, Liao S, Qi X, Liu J, Kampuansai J, Zhang H, Yang Z, Serey B, Sovannary T, Bunnath L, Aun HS, Samnom H, Kangwanpong D, Shi H, Su B (2015) Y-chromosome diversity suggests southern origin and Paleolithic backwave migration of Austro-Asiatic speakers from eastern Asia to the Indian subcontinent. Sci Rep 5:15486
Zhivotovsky LA, Underhill PA, Cinnioğlu C, Kayser M, Morar B, Kivisild T, Scozzari R, Cruciani F, Destro-Bisol G, Spedini G, Chambers GK, Herrera RJ, Yong KK, Gresham D, Tournev I, Feldman MW, Kalaydjieva L (2004) The effective mutation rate at Y chromosome short tandem repeats, with application to human population-divergence time. Am J Hum Genet 74:50–61
Zhong H, Shi H, Qi XB, Xiao CJ, Jin L, Ma RZ, Su B (2010) Global distribution of Y-chromosome haplogroup C reveals the prehistoric migration routes of African exodus and early settlement in East Asia. J Hum Genet 55:428–435
The authors thank all volunteers and village chiefs for their participation in the sample collection process. This work was financially and technically supported by the former Network of Forensic Science Institutes, Institute of Forensic Medicine, DNA laboratory (2015), Budapest, Hungary. JK gratefully acknowledges the partial support provided by Chiang Mai University, Thailand, and Tempus Public Foundation, Hungary. WK was provided funding from the Thailand Research Fund (Grant No. RSA6180058).
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General information and Fst genetic distances of Y haplogroup frequencies of 27 populations compared. Fst (below the diagonal) and their p values (above the diagonal) are shown. Grey boxes indicate non-significant p values, p>0.01 (XLSX 20 kb)
General information and Rst-genetic distances of 23 STR-based Y haplotype frequencies of 23 populations compared. Rst (below the diagonal) and their p values (above the diagonal). Grey boxes indicate non-significant p values, p>0.01 (XLSX 16 kb)
Haplotypes of 23 Y-STRs and haplogroups of 227 male samples obtained from northern Thailand (XLSX 35 kb)
Median-Joining Network (MJ) of 414 N haplotypes constructed. The circle sizes are proportional to the haplotype frequencies. The smallest area is equivalent to one individual (TIFF 8565 kb)
Median-Joining Network (MJ) of 82 G haplotypes constructed. The circle sizes are proportional to the haplotype frequencies. The smallest area is equivalent to one individual. (Arrow 1: G1 samples of Skaw and Pwo; Arrow 2: G1a common haplotype; Arrow 3: G2a common haplotype) (TIFF 60062 kb)
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Kampuansai, J., Kutanan, W., Dudás, E. et al. Paternal genetic history of the Yong population in northern Thailand revealed by Y-chromosomal haplotypes and haplogroups. Mol Genet Genomics (2020). https://doi.org/10.1007/s00438-019-01644-x
- Y haplotypes and haplogroups
- Yong ethnic groups in Thailand
- Human demographic history