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A major locus controlling malondialdehyde content under water stress is associated with Fusarium crown rot resistance in wheat

Abstract

Malondialdehyde (MDA) is a naturally occurring product of lipid peroxidation and the level of MDA in plant is often used as a parameter to evaluate the damage to plants’ cells due to stress. Plant with lower amounts of MDA under drought conditions is generally considered as more tolerant to drought. In this study, a population of recombinant inbred lines was used to map the quantitative trait locus (QTLs) that controlled MDA content under well-watered condition (WW) and water deficit (WD) condition. A major QTL, designated as Qheb.mda-3B, was detected on the long arm of chromosome 3B. Based on interval mapping analysis, Qheb.mda-3B explained 31.5 and 39.0 % of the phenotypic variance under WW and WD conditions, respectively. Qheb.mda-3B was located in the same interval as a previously identified QTL (Qcrs.cpi-3B) that controlled resistance to Fusarium crown rot (FCR), a fungal disease caused by Fusarium species. Three pairs of near-isogenic lines (NILs) previously developed for Qcrs.cpi-3B were found to show significant differences in MDA content under WD condition. These results suggested that same set of genes is likely to be involved in drought tolerance and FCR resistance in wheat.

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Abbreviations

CEF:

Controlled environment facility

MDA:

Malondialdehyde

QTL:

Quantitative trait locus

RILs:

Recombinant inbred lines

WW:

Well-watered conditions

WD:

Water deficit conditions

NILs:

Near-isogenic lines

FCR:

Fusarium crown rot

TBA:

Thiobarbituric acid

TCA:

Trichloracetic acid

MQM:

Multiple QTL model

IM:

Interval mapping

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Acknowledgments

The authors are grateful to Drs. Chunji Liu and Guijun Yan for their valuable suggestions during the preparation of the manuscript. This research was partially funded by an Australian Research Council (ARC) grant LP120200830.

Conflict of interest

J. Ma, GY. Du, XH. Li, CY. Zhang and JK. Guo declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

Author information

Correspondence to Caiying Zhang or Jinkao Guo.

Additional information

J. Ma and G. Du equally contributed to this work.

Communicated by L. Xiong.

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Ma, J., Du, G., Li, X. et al. A major locus controlling malondialdehyde content under water stress is associated with Fusarium crown rot resistance in wheat. Mol Genet Genomics 290, 1955–1962 (2015). https://doi.org/10.1007/s00438-015-1053-3

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Keywords

  • MDA content
  • QTL
  • Drought
  • Fusarium crown rot
  • Wheat