Silene latifolia is a model plant for studies of the early steps of sex chromosome evolution. In comparison to mammalian sex chromosomes that evolved 300 mya, sex chromosomes of S. latifolia appeared approximately 20 mya. Here, we combine results from physical mapping of sex-linked genes using polymerase chain reaction on microdissected arms of the S. latifolia X chromosome, and fluorescence in situ hybridization analysis of a new cytogenetic marker, Silene tandem repeat accumulated on the Y chromosome. The data are interpreted in the light of current genetic linkage maps of the X chromosome and a physical map of the Y chromosome. Our results identify the position of the centromere relative to the mapped genes on the X chromosome. We suggest that the evolution of the S. latifolia Y chromosome has been accompanied by at least one paracentric and one pericentric inversion. These results indicate that large chromosomal rearrangements have played an important role in Y chromosome evolution in S. latifolia and that chromosomal rearrangements are an integral part of sex chromosome evolution.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Atanassov I, Delichere C, Filatov DA, Charlesworth D, Negrutiu I, Moneger F (2001) Analysis and evolution of two functional Y-linked loci in a plant sex chromosome system. Mol Biol Evol 18:2162–2168
Bergero R, Forrest A, Kamau E, Charlesworth D (2007) Evolutionary strata on the X chromosomes of the dioecious plant Silene latifolia: evidence from new sex-linked genes. Genetics 175:1945–1954
Blackburn KB (1923) Sex chromosomes in plants. Nature 112:687–688
Brodie R, Roper RL, Upton C (2004) JDotter: a Java interface to multiple dotplots generated by dotter. Bioinformatics 20:279–281
Buzek J, Koutnikova H, Houben A, Riha K, Janousek B, Siroky J, Grant S, Vyskot B (1997) Isolation and characterization of X chromosome-derived DNA sequences from a dioecious plant Melandrium album. Chromosome Res 5:57–65
Charlesworth B (1991) The evolution of sex chromosomes. Science 251:1030–1033
Delichere C, Veuskens J, Hernould M, Barbacar N, Mouras A, Negrutiu I, Moneger F (1999) SlY1, the first active gene cloned from a plant Y chromosome, encodes a WD-repeat protein. EMBO J 18:4169–4179
Di Stilio VS, Kesseli RV, Mulcahy DL (1998) A pseudoautosomal random amplified polymorphic DNA marker for the sex chromosomes of Silene dioica. Genetics 149:2057–2062
Filatov DA (2005a) Evolutionary history of Silene latifolia sex chromosomes revealed by genetic mapping of four genes. Genetics 170:975–979
Filatov DA (2005b) Substitution rates in a new Silene latifolia sex-linked gene, SlssX/Y. Mol Biol Evol 22:402–408
Hobza R, Vyskot B (2007) Laser microdissection-based analysis of plant sex chromosomes. Methods Cell Biol 82:433–453
Hobza R, Lengerova M, Cernohorska H, Rubes J, Vyskot B (2004) FAST-FISH with laser beam microdissected DOP-PCR probe distinguishes the sex chromosomes of Silene latifolia. Chromosome Res 12:245–250
Hobza R, Lengerova M, Svoboda J, Kubekova H, Kejnovsky E, Vyskot B (2006a) An accumulation of tandem DNA repeats on the Y chromosome in Silene latifolia during early stages of sex chromosome evolution. Chromosoma 115:376–382
Hobza R, Hrusakova P, Safar J, Bartos J, Janousek B, Zluvova J, Michu E, Dolezel J, Vyskot B (2006b) MK17, a specific marker closely linked to the gynoecium suppression region on the Y chromosome in Silene latifolia. Theor Appl Genet 113:280–287
Kejnovsky E, Kubat Z, Hobza R, Lengerova M, Sato S, Tabata S, Fukui K, Matsunaga S, Vyskot B (2006) Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia. Genetica 128:167–175
Lahn BT, Page DC (1999) Four evolutionary strata on the human X chromosome. Science 286:964–967
Lebel-Hardenack S, Hauser E, Law TF, Schmid J, Grant SR (2002) Mapping of sex determination loci on the white campion (Silene latifolia) Y chromosome using amplified fragment length polymorphism. Genetics 160:717–725
Lengerova M, Moore RC, Grant SR, Vyskot B (2003) The sex chromosomes of Silene latifolia revisited and revised. Genetics 165:935–938
Matsunaga S, Schutze K, Donnison IS, Grant SR, Kuroiwa T, Kawano S (1999) Single pollen typing combined with laser-mediated manipulation. Plant J 20:371–378
Matsunaga S, Isono E, Kejnovsky E, Vyskot B, Dolezel J, Kawano S, Charlesworth D (2003) Duplicative transfer of a MADS box gene to a plant Y chromosome. Mol Biol Evol 20:1062–1069
Moore RC, Kozyreva O, Lebel-Hardenack S, Siroky J, Hobza R, Vyskot B, Grant SR (2003) Genetic and functional analysis of DD44, a sex-linked gene from dioecious plant Silene latifolia, provides clues to early events in sex chromosome evolution. Genetics 163:321–334
Nicolas M, Marais G, Hykelova V, Janousek B, Laporte V, Vyskot B, Mouchiroud D, Negrutiu I, Charlesworth D, Moneger F (2005) A gradual and ongoing process of recombination restriction in the evolutionary history of the sex chromosomes in dioecious plants. Public Libr Sci Biol 3:47–56
Ohno S (1967) Sex chromosomes and sex-linked genes. Springer, Berlin
Vyskot B, Hobza R (2004) Gender in plants: sex chromosomes are emerging from the fog. Trends Genet 20:432–438
Winge O (1923) On sex chromosomes, sex determination and preponderance of females in some dioecious plants. CR Trav Lab Carlsberg 15:1–26
Zluvova J, Janousek B, Negrutiu I, Vyskot B (2005) Comparison of the X and Y chromosome organization in Silene latifolia. Genetics 170:1431–1434
This research was supported by the Swiss Science Foundation (grant no. 3100AO-104114 to A.W.), and the Czech Science Foundation (grant nos. 204/05/2097 to E.K., and 521/06/0056 to B.V.).
Communicated by M.-A. Grandbastien.
About this article
Cite this article
Hobza, R., Kejnovsky, E., Vyskot, B. et al. The role of chromosomal rearrangements in the evolution of Silene latifolia sex chromosomes. Mol Genet Genomics 278, 633–638 (2007). https://doi.org/10.1007/s00438-007-0279-0
- Chromosomal rearrangements
- Sex chromosomes