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FLO11 mediated filamentous growth of the yeast Saccharomyces cerevisiae depends on the expression of the ribosomal RPS26 genes

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Abstract

The RPS26A and RPS26B isogenes of Saccharomyces cerevisiae encode two almost identical proteins of the small 40S ribosomal subunit, which differ by only two amino acid residues. Growth of an rps26BΔ mutant strain is normal, whereas an rps26AΔ strain displays a reduced growth rate and increased sensitivity towards the specific translational inhibitor paromomycin. An rps26AΔ rps26BΔ double mutant strain is inviable. RPS26A but not RPS26B is required for haploid adhesive and diploid pseudohyphal growth mediated by FLO11, which encodes an adhesion. The RPS26A and RPS26B transcripts make up about 70 and 30% of the cellular RPS26 mRNA, respectively. Overexpression of RPS26B, as well as an RPS26B open reading frame driven by the RPS26A promoter, complements the rps26AΔ deletion and restores haploid invasive growth as well as diploid pseudohyphal growth. These results suggest that the two proteins are functionally interchangeable. FLO11–lacZ activity is not present in haploid rps26AΔ yeast mutant strains, even though FLO11 mRNA levels are not reduced. This suggests that the amount of Rps26p is critical for accurate translation of the FLO11 mRNA, and therefore for the dimorphic switch of the bakerȁ9s yeast from a single cell yeast to an adhesive filamentous growth form.

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Acknowledgements

We thank Sven Krappmann and Hans-Ulrich Mösch for their ideas and their support during the entire study period. We are grateful to Maria Meyer for her help in the tetrad dissection and to Brita Kotrasch and Elizabeth Dennison for critically reading of the manuscript. Furthermore, we would like to thank all members of our group for their helpful discussions. This work was supported by grants from the Deutsche Forschungsgemeinschaft, the Volkswagen-Stiftung and the Fonds der Chemischen Industrie.

Author information

Correspondence to Gerhard H. Braus.

Additional information

Axel W. Strittmatter and Claudia Fischer have contributed equally to this work.

Communicated by S. Hohmann

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Strittmatter, A.W., Fischer, C., Kleinschmidt, M. et al. FLO11 mediated filamentous growth of the yeast Saccharomyces cerevisiae depends on the expression of the ribosomal RPS26 genes. Mol Genet Genomics 276, 113–125 (2006). https://doi.org/10.1007/s00438-006-0127-7

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Keywords

  • Saccharomyces cerevisiae
  • Rps26p
  • FLO11
  • Adhesion
  • Pseudohyphae