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Depletion of Aspergillus nidulans cotA causes a severe polarity defect which is not suppressed by the nuclear migration mutation nudA2

Abstract

The Aspergillus nidulans homologue of Neurospora crassa cot-1, cotA, encoding a member of the NDR protein kinase family, has been cloned and expressed under the control of the conditional alcA promoter. Depletion of CotA by repression of the alcA promoter led to a severe growth defect accompanied by loss of polarity. Germlings show greatly enlarged volume of the spores and hyphae, accompanied by an increase in number of nuclei per compartment, though the nucleus/volume ratio is not significantly altered. The depleted CotA phenotype was not suppressed by a nuclear migration mutation nudA2. Double mutants showed an additive, defective phenotype, unlike the suppression of the cot-1 ts mutation by ropy mutations seen in N. crassa, suggesting a different relationship between nuclear migration and the cot signalling pathway in A. nidulans. A functional CotA–GFP fusion protein was found in punctate regions of fluorescence similar to the distribution reported for human NDR2, and as a cap at the hyphal tip.

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Acknowledgements

This work was supported by BBSRC grant 50/G06623 to GT, BBSRC studentships to SJ and ACL and a Ministry of Culture and Higher Education of Iran studentship to MS. We thank Mrs. J. Price and Ms. N. Frima for technical assistance. All genetic manipulation experiments were carried out in accordance with local regulations.

Author information

Correspondence to Geoffrey Turner.

Additional information

Communicated by P. Punt.

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Johns, S.A., Leeder, A.C., Safaie, M. et al. Depletion of Aspergillus nidulans cotA causes a severe polarity defect which is not suppressed by the nuclear migration mutation nudA2 . Mol Genet Genomics 275, 593 (2006). https://doi.org/10.1007/s00438-006-0113-0

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Keywords

  • Aspergillus nidulans
  • Polarity
  • Protein kinase
  • GFP
  • Nuclear migration