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Kinase Cak1 functionally interacts with the PAF1 complex and phosphatase Ssu72 via kinases Ctk1 and Bur1

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Abstract

Protein kinases orthologous with Cak1 of Saccharomyces cerevisiae (ScCak1) appear specific to ascomycetes. ScCak1 phosphorylates Cdc28, the cyclin-dependent kinase (CDK) governing the cell cycle, as well as Kin28, Bur1 and Ctk1, CDKs required for the transcription process performed by RNA polymerase II (RNA Pol II). Using genetic methods, we found that Cak1 genetically interacts with Paf1 and Ctr9, two components belonging to the PAF1 elongation complex needed for histone modifications, and with Ssu72, a protein phosphatase that dephosphorylates serine-5 phosphate in the RNA Pol II C-terminal domain. We present evidence suggesting that the interactions linking Cak1 with the PAF1 complex and with Ssu72 are not direct but mediated via Ctk1 and Bur1. We discuss the possibility that Ssu72 intervenes at the capping checkpoint step of the transcription cycle.

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Acknowledgments

We are grateful to V. Goguel, F. Cross, D. Morgan and G. Prelich for plasmids and materials; J.B. Weitzman, Y. Cohen and R. Chanet for useful suggestions; and M. Pierre for excellent secretarial assistance. C. Miled was supported by a fellowship from the Association pour la Recherche sur le Cancer.

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Correspondence to Gérard Faye.

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Article note

Carine Ganem and Chaouki Miled contributed equally to this work.

Communicated by A. Aguilera

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Ganem, C., Miled, C., Facca, C. et al. Kinase Cak1 functionally interacts with the PAF1 complex and phosphatase Ssu72 via kinases Ctk1 and Bur1. Mol Genet Genomics 275, 136–147 (2006). https://doi.org/10.1007/s00438-005-0071-y

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Keywords

  • Cak1
  • Paf1 complex
  • Phosphatase Ssu72
  • Ctk1
  • Bur1