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Ectopic expression of the Suppressor of Underreplication gene inhibits endocycles but not the mitotic cell cycle in Drosophila melanogaster

Abstract

The Suppressor of Underreplication ( SuUR) gene contributes to the regulation of DNA replication in regions of intercalary heterochromatin in salivary gland polytene chromosomes. In the SuUR mutant these regions complete replication earlier than in wild type and, as a consequence, undergo full polytenization. Here we describe the effects of ectopic expression of SuUR using the GAL4-UAS system. We demonstrate that ectopically expressed SuUR exerts qualitatively distinct influences on polyploid and diploid tissues. Ectopic expression of SuUR inhibits DNA replication in polytene salivary gland nuclei, and reduces the degree of amplification of chorion protein genes that occurs in the follicle cell lineage. Effects caused by ectopic SuUR in diploid tissues vary considerably; there is no obvious effect on eye formation, but apoptosis is observed in the wing disc, and wing shape is distorted. The effect of ectopic SuUR expression is enhanced by mutations in the genes E2F and mus209 ( PCNA). Differential responses of polyploid and diploid cells to ectopic SuUR may reflect differences in the mechanisms underlying mitotic cell cycles and endocycles.

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Acknowledgements

The authors are grateful to Gines Morata, Lynn Manseau, Carole Seum, Francois Karch, Yuri Moshkin and Bloomington Drosophila Stock Center for providing fly stocks. The work was supported by grants from the program "Frontiers in Genetics" of the Russian Federation (Grant 2-02 PNG), the RFBR program 02-04-48222 and the program for Physical-Chemical Biology. The experiments comply with the current laws of the Russian Federation.

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Correspondence to I. F. Zhimulev.

Additional information

Communicated by G. P. Georgiev

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Volkova, E.I., Yurlova, A.A., Kolesnikova, T.D. et al. Ectopic expression of the Suppressor of Underreplication gene inhibits endocycles but not the mitotic cell cycle in Drosophila melanogaster . Mol Genet Genomics 270, 387–393 (2004). https://doi.org/10.1007/s00438-003-0924-1

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Keywords

  • Drosophila
  • Endocycle
  • Suppression of DNA replication