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Mapping of gene-specific markers on the genetic map of chickpea ( Cicer arietinum L.)

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Abstract

With the exception of the fact that it is made up of eight different chromosomes, the physical organization of the 738-Mb genome of the important legume crop chickpea (Cicer arietinum L.) is unknown. In an attempt to increase our knowledge of the basic structure of this genome, we determined the map positions of a series of genes involved in plant defence responses (DR) by genetic linkage analysis. Exploiting the sequence data available in GenBank, we selected genes known to be induced in chickpea and other plants by pathogen attack. Gene-specific primers were designed based on conserved regions, and used to detect the corresponding gene sequences in a segregating population derived from an interspecific cross between Cicer arietinum and C. reticulatum. Forty-seven gene-specific markers were integrated into an existing map based on STMS, AFLP, DAF and other anonymous markers. The potential of this approach is discussed.

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Acknowledgements

This research was supported by grants from the BMZ, Bonn, Germany (Grant 89) and Deutsche Forschungsgemeinschaft (Grant Ka 332/17-1-3). The senior author appreciates the generosity of his colleagues from the Botany Department in allowing him to use the laboratory facilities for this work after his retirement.

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Correspondence to G. Kahl.

Additional information

Communicated by R. Hagemann

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Pfaff, T., Kahl, G. Mapping of gene-specific markers on the genetic map of chickpea ( Cicer arietinum L.). Mol Gen Genomics 269, 243–251 (2003). https://doi.org/10.1007/s00438-003-0828-0

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Keywords

  • Chickpea
  • Plant defence genes
  • Genetic markers
  • Mapping