Information about genetic changes during the selection process could indicate mechanisms underlying the spread of resistance to anthelmintic drugs. For clarification of the role of the Mpi locus encoding mannose-phosphate isomerase enzyme in determining resistance, genotyping of Oesophagostomum dentatum strains was performed using an isoelectrofocusing technique. In levamisole- and pyrantel-selected strains the allele associated with resistance has probably been found. Significant values for genetic differentiation between treated and untreated strains of common origin were recorded by Fst indices (θ=0.078; P=0.0008). The specific genomic makeup of a flubendazole-resistant strain, which did not correlate with that of the remaining isolates, might be ascribed to a different action of the anthelmintic or different environmental conditions under which resistance against this drug arose. The absence of heterozygotes in male populations indicated an XX/X0 system of sex determination for the Mpi locus, thus providing a greater potential for the development of resistance. A possible involvement of alleles linked with mannose-phosphate isomerase in alterations of membrane receptors that can be associated with resistance against imidothiazole-based drugs is discussed.
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Received: 24 September 1999 / Accepted: 24 November 1999
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Šnábel, V., DeMeeŵs, T., Várady, M. et al. The sexually linked Mpi locus is presumably involved in imidothiazole resistance in Oesophagostomum dentatum parasites. Parasitol Res 86, 486–490 (2000). https://doi.org/10.1007/s004360050698
- Selection Process
- Genetic Differentiation
- Genetic Change
- Membrane Receptor