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Effects of temperature and white sucker (Catostomus commersoni) serum supplement on the in vitro multiplication of Cryptobia catostomi in cell-free culture medium

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Abstract

Cryptobia catostomi, a non-pathogenic haemoflagellate of white suckers (Catostomus commersoni) multiplied rapidly in modified TDL-15 medium supplemented with 10% fetal bovine serum and 1% heat-inactivated white sucker serum (WSS) at 10°C and 18°C. The numbers of C. catostomi counted were significantly higher in cultures incubated at 10°C than in those incubated at 18°C beginning at 3 weeks post-incubation. The culture forms (from the eighth subculture at 9 months after isolation) were morphologically similar to blood forms and were infective to laboratory-raised white suckers. The parasite survived for about 4 weeks in the medium without WSS at 18°C. The present study indicates that WSS supplement supports the in vitro multiplication of C. catostomi and that its multiplication is temperature-dependent.

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Received: 24 May 1995 / Accepted: 18 October 1995

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Li, S., Woo, P. Effects of temperature and white sucker (Catostomus commersoni) serum supplement on the in vitro multiplication of Cryptobia catostomi in cell-free culture medium. Parasitol Res 82, 276–278 (1996). https://doi.org/10.1007/s004360050112

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Keywords

  • Bovine Serum
  • Fetal Bovine Serum
  • Serum Supplement
  • Culture Form
  • White Sucker