Cryptobia catostomi, a non-pathogenic haemoflagellate of white suckers (Catostomus commersoni) multiplied rapidly in modified TDL-15 medium supplemented with 10% fetal bovine serum and 1% heat-inactivated white sucker serum (WSS) at 10°C and 18°C. The numbers of C. catostomi counted were significantly higher in cultures incubated at 10°C than in those incubated at 18°C beginning at 3 weeks post-incubation. The culture forms (from the eighth subculture at 9 months after isolation) were morphologically similar to blood forms and were infective to laboratory-raised white suckers. The parasite survived for about 4 weeks in the medium without WSS at 18°C. The present study indicates that WSS supplement supports the in vitro multiplication of C. catostomi and that its multiplication is temperature-dependent.
This is a preview of subscription content, log in to check access.
We’re sorry, something doesn't seem to be working properly.
Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.
Received: 24 May 1995 / Accepted: 18 October 1995
About this article
Cite this article
Li, S., Woo, P. Effects of temperature and white sucker (Catostomus commersoni) serum supplement on the in vitro multiplication of Cryptobia catostomi in cell-free culture medium. Parasitol Res 82, 276–278 (1996). https://doi.org/10.1007/s004360050112
- Bovine Serum
- Fetal Bovine Serum
- Serum Supplement
- Culture Form
- White Sucker