Intracellular iron homeostasis of vertebrates and invertebrates is mediated through the interaction of iron-regulatory proteins (IRPs) with mRNAs containing a bulged hairpin-loop structure termed the iron-responsive element (IRE). We detected a protein within extracts prepared from Leishmania tarentolae that specifically interacts with a mammalian IRE; mutations to the IRE that inhibit the interaction with the mammalian protein have a corresponding effect on the interaction with the L. tarentolae protein. The disassociation constant noted for the interaction of the mammalian IRE with the L. tarentolae protein was 0.7 ± 0.3 μM, whereas that recorded for the interaction with the mammalian IRP under these conditions was 5 ± 2 nM. The interacting L. tarentolae protein potentially places the RNA-binding site of the IRP near the root of the eukaryotic evolutionary tree. However, unlike that of the mammalian IRPs, the L. tarentolae IRE-binding activity was not induced by growth in iron-depleted media.
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Received: 21 June 1999 / Accepted: 15 September 1999
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Meehan, H., Lundberg, R. & Connell, G. A trypanosomatid protein specifically interacts with a mammalian iron-responsive element. Parasitol Res 86, 109–114 (2000). https://doi.org/10.1007/s004360050019
- Evolutionary Tree
- Iron Homeostasis
- Mammalian Protein
- Intracellular Iron