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Parasitology Research

, Volume 118, Issue 12, pp 3443–3447 | Cite as

Molecular characterization of Eimeria sp. from Galápagos giant tortoises (Chelonoidis spp.)

  • G. Sevillano
  • W. Tapia
  • A. Loyola
  • A. Reyna-Bello
  • Freddy Proaño-PérezEmail author
Protozoology - Original Paper
  • 36 Downloads

Abstract

Galápagos giant tortoises are an essential component of their ecosystem and evaluation of parasites in their populations is essential for the management of conservation processes. Coccidiosis is the most common intestinal infection in free-living and captive reptiles. The aim of this study was to characterize molecularly the presence of Eimeria sp. in captive reared giant tortoises from Santa Cruz, Santiago, Española, and Pinzon Islands hatched and housed at the tortoise rearing center on Santa Cruz Island, Galápagos, by sequencing of the 18S rRNA gene. Galápagos. All samples were previously analyzed by coproparasitoscopic flotation technique and PCR for molecular identification. The results obtained by microscopy examination showed oocysts in all samples. PCR and sequencing indicated the presence Eimeria sp., showing a similarity percentage of 98% with Eimeria environmental. In conclusion, we identified a group of coccidia of the genus Eimeria sp. (MK909931) in Galápagos tortoises.

Keywords

Molecular characterization Eimeria sp. Giant tortoises Chelonoidis spp. Galápagos 

Notes

Acknowledgments

Special thanks to the Galápagos National Park Directorate for permitting this study, and the Laboratory of Animal Biotechnology, Universidad de las Fuerzas Armadas (ESPE) for their provision of the reagents and the use of equipment.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Grupo de Investigación en Sanidad Animal y Humana (GISAH), Departamento de Ciencias de la Vida y la AgriculturaUniversidad de las Fuerzas Armadas ESPELatacungaEcuador
  2. 2.Galápagos ConservancyFairfaxUSA
  3. 3.Parque Nacional GalápagosPuerto AyoraEcuador
  4. 4.Facultad de Medicina Veterinaria y ZootecniaUniversidad Central del EcuadorQuitoEcuador
  5. 5.Department of Life Sciences and AgricultureUniversidad de las Fuerzas Armadas ESPESangolquíEcuador

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