Lipase is associated with deltamethrin resistance in Culex pipiens pallens

  • Hong-Xia Hu
  • Dan Zhou
  • Lei Ma
  • Bo Shen
  • Yan SunEmail author
  • Chang-Liang Zhu
Arthropods and Medical Entomology - Original Paper


The wide application of pyrethroids has led to the rapid development of insecticide resistance in mosquitoes, leading to a rise in mosquito-borne diseases. We previously identified five differentially expressed lipase family genes upon evaluating the transcriptomes of deltamethrin-resistant and deltamethrin-susceptible strains of Culex pipiens pallens. Herein, the gene expression levels were verified by quantitative real-time PCR, and two lipase family genes, lipase A and pancreatic triacylglycerol lipase A, were chosen for further investigations. Using cell viability assays and Centers for Disease Control and Prevention bottle bioassays, lipase A was found to increase the resistance of mosquitoes against deltamethrin both in vitro and in vivo. Our findings indicate that lipase A is involved in conferring deltamethrin resistance in Cx. pipiens pallens.


Lipase Deltamethrin Insecticide resistance Culex pipiens pallens 


Author contributions

HXH performed the experiments. HXH and DZ wrote the manuscript and prepared the figures. DZ, LM, and BS participated in data analyses. YS and CLZ conceived the idea and coordinated the project. All authors have read and approved the final version of the manuscript for submission.

Funding information

This study was supported by the National Natural Science Foundation of China (Grant No. 81772227, 81672056, and 81672058) and the National S & T Major Program (Grant No. 2017ZX10303404-002-006).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Not applicable

Supplementary material

436_2019_6489_MOESM1_ESM.docx (40 kb)
ESM 1 (DOCX 40 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pathogen BiologyNanjing Medical UniversityNanjingChina
  2. 2.Department of Clinical Medical LaboratoryThe First Affiliated Hospital, and College of Clinical Medicine of Henan University of Science and TechnologyLuoyangChina

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