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De novo transcriptome sequencing and differential gene expression analysis of two parasitic human Demodex species

  • Li Hu
  • Yae ZhaoEmail author
  • Dongling Niu
  • Xiaojuan Gong
  • Rui Yang
Arthropods and Medical Entomology - Original Paper

Abstract

Demodex are among the tiniest organisms in Acari and are important mammalian parasites. However, differences in pathogenicity between two human parasites, Demodex folliculorum and Demodex brevis, remain unknown. Related genetic studies are limited by RNA extraction difficulties and molecular data deficiencies. In this study, RNA extraction, de novo sequencing, functional annotation, and differential gene expression analyses were performed to compare D. folliculorum and D. brevis. This yielded 67.09 and 65.10 million clean reads, respectively, with similar annotations. Bioinformatics analyses and manual alignments identified 237 coding sequences comprising 48 genes from 29 families, including five important functional classes. Of these, 30 genes from 20 families related to metabolism, motion, detoxification and stress response, and allergic reaction were differentially expressed between the two species. Cathepsin type 1, serine protease inhibitor, arginine kinase, triosephosphate isomerase, muscle-specific protein 20-2, myosin alkaline light chain, troponin C, tropomyosin, and heat shock protein 90 were highly expressed in D. folliculorum, whereas cathepsin type 2, aspartic protease, serine protease, myosin heavy chain type 2, and alpha tubulin type 1C were highly expressed in D. brevis. Verified coding sequences were nearly consistent with unigene clusters. Further, absolute quantification results demonstrated that differentially expressed genes followed the predicted expression trend. Therefore, the first RNA sequencing and functional annotation analysis of two Demodex species was successful. Differential expression of important functional genes is likely implicated in pathogenicity disparities between these two species. Our study provides molecular data and technical support for further studies on human Demodex pathogenicity and functional genes.

Keywords

Human Demodex Pathogenicity differences de novo transcriptome sequencing Differentially expressed gene screening Expression quantity verification 

Notes

Funding information

This work was supported by National Natural Science Foundation of China (Nos. 81471972 and 81271856).

Compliance with ethical standards

The present study was approved by the Ethics Committee of Medical and Biological Research of Xi’an Jiaotong University Health Science Center (approval no. 2019-002). All subjects were sampled by the authors or associated project staff. Written informed consent was obtained from all patients.

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Li Hu
    • 1
  • Yae Zhao
    • 1
    Email author
  • Dongling Niu
    • 1
  • Xiaojuan Gong
    • 1
  • Rui Yang
    • 1
  1. 1.Department of Pathogen Biology and Immunology, School of Basic Medical SciencesXi’an Jiaotong UniversityXi’anChina

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