Diversity of Empruthotrema Johnston and Tiegs, 1992 parasitizing batoids (Chondrichthyes: Rajiformes and Myliobatiformes) from the Southwest Atlantic Ocean, with description of three new species

  • Manuel M. IrigoitiaEmail author
  • Paola E. Braicovich
  • María A. Rossin
  • Delfina Canel
  • Eugenia Levy
  • Marisa D. Farber
  • Juan T. Timi
Fish Parasitology - Original Paper


During an extensive research project involving 519 specimens of batoids, including 13 species of Rajiformes and Myliobatiformes (Chondrichthyes) from the Argentine Sea, three new species of Empruthotrema were found and are described using morphologic characteristics and two molecular markers: LSU rDNA and COI mtDNA. The new species can be distinguished from their congeners by the number and distribution of the marginal loculi, the length and morphology of male copulatory organ, and the presence of eyespots. Additionally, multivariate analysis identified the dimensions of the pharynx and ejaculatory bulb as diagnostic features. Host specificity and previous records of the genus in the region are discussed. This is the first description of new species in this genus for the Southwestern Atlantic Ocean, as well as for arhynchobatid hosts.


Empruthotrema aoneken Empruthotrema orashken Empruthotrema dorae Rajiformes Myliobatiformes Argentine Sea 



Thanks are extended to Dr. Aneta Yoneva from the Institute of Biodiversity and Ecosystem Research (Sofia, Bulgaria) for translating Russian literature. To Dr. Ana Fernandez Garay from the Instituto de Lingüística y Letras UBA-CONICET (Argentina) for her help with Tehuelche native language. To Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP) and Industrias El Corsario A.A. (Mar del Plata, Argentina), for providing fish samples. To Dr. Santiago Barbini, Dr. David Sabadin, Lic. Jorge Roman, Lic. Melisa Chierichietti and Dr. Lorena Scenna (IIMyC-CONICET) for helping with fish identification and sample collection.

Funding information

Financial support provided by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (PIP No. 112-201501-00973), Fondo para la Investigación Científica y Tecnológica (PICT 2015 No. 2013) and Universidad Nacional de Mar del Plata (EXA 915/18).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratorio de Ictioparasitología, Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Marinas y Costeras (IIMyC)Universidad Nacional de Mar del Plata (UNMdP)Buenos AiresArgentina
  2. 2.Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA)Buenos AiresArgentina

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