Immunodiagnosis of human neurocysticercosis: comparative performance of serum diagnostic tests in Mexico
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Immunodiagnosis has a supportive role in the diagnosis of neurocysticercosis (NCC). The aim of this study was to compare the validity of seven immunodiagnostic tests among serum samples from 58 patients with NCC, 26 patients with neurological diseases other than NCC, and 15 healthy controls. One test for viable parasite detection (HP10 antigen assay) and six for antibody detection were evaluated. For the entire sample, sensitivities ranged from 55.2% (NOVALISA) to 81.0% (enzyme-linked immunosorbent assay [ELISA] Taenia solium antibody), with the sensitivity of the latter test significantly higher than that of the in-house ELISA Taenia crassiceps, NOVALISA, enzyme-linked immunoelectrotransfer blot (EITB) CDC, and HP10. Overall, specificities were high, ranging from 85.4% (ELISA Ts) to 97.1% (NOVALISA), with no statistically significant differences. Detection of HP10 antigen was significantly associated with the presence of vesicular parasites. The simple and low-cost ELISA Taenia solium antibody Ab instead of EITB is recommended to support NCC diagnosis in both rural and hospital settings in Mexico.
KeywordsELISA EITB HP10 Immunodiagnosis Mexico Neurocysticercosis
This study was designed, supervised, and coordinated by RMEP, TG, ES, and AF. MH, OGA, GD, JLRA, AML, RGR, and AT carried out the clinical assessment and the immunoassays; MLR and AF carried out the analysis and interpretation of these data. JLRA, RMEP, ES, and AF drafted the manuscript. All authors critically revised the manuscript for intellectual content, read, and approved the final manuscript. ES and AF are guarantors of the paper.
This work was partially supported by the “Programa Iberoamericano de Ciencia y TecnologÍa para el Desarrollo, CYTED” [211RT0432] and by the “Programa de Investigación para el Desarrollo y la Optimización de Vacunas, Inmunomoduladores y Métodos Diagnósticos del IIB” (PROVACADI, Instituto de Investigaciones Biomédicas, UNAM, México).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Bobes RJ, Hernández M, Márquez C, Fragoso G, García E, Parkhouse RM, Harrison LJ, Sciutto E, Fleury A (2006) Subarachnoidal and intraventricular human neurocysticercosis: application of an antigen detection assay for the diagnosis and follow-up. Tropical Med Int Health 11:943–950CrossRefGoogle Scholar
- Brandt JRA, Geerts S, De Deken R, Kumar V, Ceulemans F, Brijs L, Falla N (1992) A monoclonal antibody-based ELISA for the detection of circulating excretory-secretory antigens in Taenia saginata cysticercosis. Int J Parasitol 22:471–477. https://doi.org/10.1016/0020-7519(92)90148-E CrossRefGoogle Scholar
- Cardona-Arias JA, Carrasquilla-Agudelo YE, Restrepo-Posada DC (2017) Validity of three methods for inmuno-diagnostic of neurocysticercosis: systematic review of the literature with meta-analysis 1960-2014. Rev Chil Infectol 34:33–44. https://doi.org/10.4067/S0716-10182017000100005 CrossRefGoogle Scholar
- Carod JF, Randrianarison M, Razafimahefa J, Mamitiana Ramahefarisoa R, Rakotondrazaka M, Debruyne M, Dautigny M, Cazalf P, Lalaoarisoa Andriantseheno M, Ramarokoto Charles E (2012) Evaluation of the performance of 5 commercialized enzyme immunoassays for the detection of Taenia solium antibodies and for the diagnosis of neurocysticercosis. Diagn Microbiol Infect Dis 72:85–89. https://doi.org/10.1016/j.diagmicrobio.2011.09.014 CrossRefGoogle Scholar
- Carpio A, Fleury A, Romo ML, Abraham R, Fandiño J, Durán JC, Cárdenas G, Moncayo J, Leite Rodrigues C, San-Juan D, Serrano-Dueñas M, Takayanagui O, Sander JW (2016) New diagnostic criteria for neurocysticercosis: reliability and validity. Ann Neurol 80:434–442. https://doi.org/10.1002/ana.24732 CrossRefGoogle Scholar
- Fleury A, Hernández M, Avila M, Cárdenas G, Bobes RJ, Huerta M, Fragoso G, Uribe-Campero L, Harrison LJ, Parkhouse RM, Sciutto E (2007) Detection of HP10 antigen in serum for diagnosis and follow-up of subarachnoidal and intraventricular human neurocysticercosis. J Neurol Neurosurg Psychiatry 78:970–974CrossRefGoogle Scholar
- Garcia HH, Castillo Y, Gonzales I, Bustos JA, Saavedra H, Jacob L, Del Brutto OH, Wilkins PP, Gonzalez AE, Gilman RH, Cysticercosis Working Group in Peru (2018b) Low sensitivity and frequent cross-reactions in commercially available antibody detection ELISA assays for Taenia solium cysticercosis. Tropical Med Int Health 23:101–105. https://doi.org/10.1111/tmi.13010 CrossRefGoogle Scholar
- Harrison LJS, Joshua GWP, Wright SH, Parkhouse RME (1989) Specific detection of circulating surface/secreted glycoproteins of viable cysticerci in Taenia saginata cysticercosis. Parasite Immunol 11:351–370. https://doi.org/10.1111/j.1365-3024.1989.tb00673.x CrossRefGoogle Scholar
- Maravilla P, Gonzalez-Guzman R, Zuñiga G, Peniche A, Dominguez-Alpizar JL, Reyes-Montes R, Flisser A (2008) Genetic polymorphism in Taenia solium cysticerci recovered from experimental infections in pigs. Infect Genet Evol 8:213–216. https://doi.org/10.1016/j.meegid.2007.11.006 CrossRefGoogle Scholar
- Ramos-Kuri M, Montoya RM, Padilla A, Govezensky T, Díaz ML, Sciutto E, Sotelo J, Larralde C (1992) Immunodiagnosis of neurocysticercosis. Disappointing performance of serology (enzyme-linked immunosorbent assay) in an unbiased sample of neurological patients. Arch Neurol 49:633–636CrossRefGoogle Scholar
- Torgerson PR, Devleesschauwer B, Praet N, Speybroeck N, Willingham AL, Kasuga F, Rokni MB, Zhou XN, Fèvre EM, Sripa B, Gargouri N, Fürst T, Budke CM, Carabin H, Kirk MD, Angulo FJ, Havelaar A, de Silva N (2015) World Health Organization estimates of the global and regional disease burden of 11 foodborne parasitic diseases, 2010: a data synthesis. PLoS Med 12:e1001920. https://doi.org/10.1371/journal.pmed.1001920 CrossRefGoogle Scholar
- Vega R, Piñero D, Ramanankandrasana B, Dumas M, Bouteille B, Fleury A, Sciutto E, Larralde C, Fragoso G (2003) Population genetic structure of Taenia solium from Madagascar and Mexico: implications for clinical profile diversity and immunological technology. Int J Parasitol 33:1479–1485CrossRefGoogle Scholar
- Zea-Vera A, Cordova EG, Rodriguez S, Gonzales I, Pretell EJ, Castillo Y, Castro-Suarez S, Gabriël S, Tsang VC, Dorny P, Garcia HH, Cysticercosis working group in Peru (2013) Parasite antigen in serum predicts the presence of viable brain parasites in patients with apparently calcified cysticercosis only. Clin Infect Dis 57:e154–e159. https://doi.org/10.1093/cid/c CrossRefGoogle Scholar