Neurocysticercosis serodiagnosis: mimotope-based synthetic peptide as potential biomarker
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Herein, we evaluate a mimotope-based synthetic peptidenamed NC41 to diagnose neurocysticercosis (NC), a neglected parasitic disease and a major cause of epilepsy worldwide. NC41 synthetic peptide was evaluated to diagnose NC, and total saline extract from Taenia solium metacestodes (SE) was used as control. Serum samples from patients with NC (n = 40), other parasitic diseases (n = 43), and healthy individuals (n = 40) were tested. Diagnostic parameters such as sensitivity (Se), specificity (Sp), likelihood ratio (LR), and area under curve (AUC) were calculated using receiver operating characteristic (ROC) curves. The sequence from T. solium phosphoenolpyruvate carboxykinase (PEPCK) was used for epitope prediction, resulting in one high-scoring patch centered at residue L247. NC41 synthetic peptide reached high diagnostic performance (Se 97.5% and Sp 97.5%, LR+ 39 and AUC 0.997). Data from diagnostic parameters and in silico analyses proved the usefulness of NC41 synthetic peptide as a diagnostic marker for human NC.
KeywordsELISA Epitope Prediction NC41 Serodiagnosis Synthetic peptide
This study was supported by the Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES—no. 3200601004M-8), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG—no. CBB-PPM-00396-13), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq—no. 302426/2012-4).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in studies involving human participants were in accordance with the ethical standards of the Research Ethics Committee of the Universidade Federal de Uberlândia (UFU), Minas Gerais state, Brazil (approved under protocol number 041/09), and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
- Elisei RMT, Matos CS, Carvalho AMRS, Chaves AT, Medeiros FAC, Barbosa R, Marcelino AP, dos Santos Emidio K, Coelho EAF, Duarte MC, de Oliveira Mendes TA, da Costa Rocha MO, Menezes-Souza D (2018) Immunogenomic screening approach to identify new antigens for the serological diagnosis of chronic Chagas’ disease. Appl Microbiol Biotechnol 102:6069–6080. https://doi.org/10.1007/s00253-018-8992-7 CrossRefGoogle Scholar
- FAO/WHO [Food and Agriculture Organization of the United Nations/World Health Organization] (2014) Multicriterial-based ranking for risk management of food-borne parasites. Microbiologycal Risk Assessment Series No 23. Rome. 302ppGoogle Scholar
- Hernández-González A, Noh J, Perteguer MJ, Gárate T, Handali S (2017) Comparison of T24H-his, GST-T24H and GST-Ts8B2 recombinant antigens in western blot, ELISA and multiplex bead-based assay for diagnosis of neurocysticercosis. Parasit Vectors 10:237–248. https://doi.org/10.1186/s13071-017-2160-2 CrossRefPubMedPubMedCentralGoogle Scholar
- Lage DP, Martins VT, Duarte MC, Costa LE, Garde E, Dimer LM, Kursancew AC, Chávez-Fumagalli MA, de Magalhães-Soares DF, Menezes-Souza D, Roatt BM, Machado-de-Ávila RA, Soto M, Tavares CA, Coelho EA (2016) A new Leishmania-specific hypothetical protein and its non-described specific B cell conformational epitope applied in the serodiagnosis of canine visceral leishmaniasis. Parasitol Res 115:1649–1658. https://doi.org/10.1007/s00436-016-4904-x CrossRefPubMedGoogle Scholar
- Leung NY, Wai CY, Ho MH, Liu R, Lam KS, Wang JJ, Shu SA, Chu KH, Leung PS (2017) Screening and identification of mimotopes of the major shrimp allergen tropomyosin using one-bead-one-compound peptide libraries. Cell Mol Immunol 14:308–318. https://doi.org/10.1038/cmi.2015.83 CrossRefPubMedGoogle Scholar
- Noh J, Rodriguez S, Lee YM, Handali S, Gonzalez AE, Gilman RH, Tsang VC, Garcia HH, Wilkins PP (2014) Recombinant protein and synthetic peptide-based immunoblot test for diagnosis of neurocysticercosis. J Clin Microbiol 52:1429–1434. https://doi.org/10.1128/JCM.03260-13 CrossRefPubMedPubMedCentralGoogle Scholar
- Sotelo J, Guerrero V, Rubio F (1985) Neurocysticercosis: a new classification based on active and inactive forms. A study of 753 cases. Arch Intern Med 145:442–445. https://doi.org/10.1001/archinte.1985.00360030074016 CrossRefPubMedGoogle Scholar