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Molecular characterization of Blastocystis in cattle in Turkey

  • Zeynep Erdem Aynur
  • Özgür GüçlüEmail author
  • İbrahim Yıldız
  • Hüseyin Aynur
  • Hatice Ertabaklar
  • Bülent Bozdoğan
  • Sema Ertuğ
Genetics, Evolution, and Phylogeny - Short Communication

Abstract

Blastocystis genus exist in a wide variety of hosts, including humans, birds, insects, annelids, amphibians, fish, and mammals. PCR-based molecular diagnostic methods have been successfully used to detect Blastocystis spp. in feces, and small subunit ribosomal ribonucleic acid (SSU rRNA) gene-based subtyping is the preferred method for diagnosis. There has been discussion about the subtypes of Blastocystis spp. which has been detected so far. To date, 26 different subtypes have been reported. The aim of this study was to determine the existence and diversity of Blastocystis spp. in cattle. In our study, a total of 80 stool samples were collected from cows and calves at 13 different farms in Burdur and one farm in Aydın. Using molecular method, a total of 9 samples out of 80 samples were found to be positive (11.25%) for Blastocystis. As a result of sequence analysis of Blastocystis positive samples, the subtype 14 was detected on seven samples, while in the other two samples, Blastocystis subtype 10 was identified. The ST10 and ST14 subtypes are commonly reported in animals but not isolated from human. Our analyses showed genetic differences among Blastocystis subtypes. Our study is the first Blastocystis subtyping study from cattle in Turkey.

Keywords

Blastocystis Cattle SSU rRNA Turkey 

Notes

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Zeynep Erdem Aynur
    • 1
  • Özgür Güçlü
    • 1
    • 2
    Email author
  • İbrahim Yıldız
    • 3
  • Hüseyin Aynur
    • 4
  • Hatice Ertabaklar
    • 3
  • Bülent Bozdoğan
    • 1
    • 5
  • Sema Ertuğ
    • 3
  1. 1.Recombinant DNA and Recombinant Protein Research Center (REDPROM)Adnan Menderes UniversityAydınTurkey
  2. 2.Department of Plant and Animal Production, Sultanhisar MYOAdnan Menderes UniversityAydınTurkey
  3. 3.Department of Parasitology, Faculty of MedicineAdnan Menderes UniversityAydınTurkey
  4. 4.Yore Veterinary ClinicAydınTurkey
  5. 5.Department of Medical Microbiology, Faculty of MedicineAdnan Menderes UniversityAydınTurkey

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