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Phylogenetic analysis of Theileria equi and Babesia caballi sequences from thoroughbred mares and foals in Trinidad

  • Candice SantEmail author
  • Orchid M. Allicock
  • Roger d’Abadie
  • Roxanne Albertha Charles
  • Karla Georges
Genetics, Evolution, and Phylogeny - Original Paper

Abstract

The agents of equine piroplasmosis, Theileria equi and Babesia caballi, are endemic in Trinidad, West Indies. While transmission is mainly by ixodid ticks, transplacental transmission of T. equi has also been reported. This disease has contributed to foetal losses as well as morbidity and mortality of neonatal foals and adult horses. Previous 18S rRNA–based phylogenetic studies indicated a noticeable degree of variation within and among B. caballi and T. equi isolates from different geographical regions. The objective of this study was to evaluate the diversity of T. equi and B. caballi obtained from horses in Trinidad by amplifying a region of the 18S rRNA gene. The phylogenetic trees for T. equi sequences obtained from horses in 2006 and 2011–2013 revealed that Trinidad sequences were of genotype A. Additionally, all of the B. caballi sequences from Trinidad were grouped together with other B. caballi sequences of genotype A. However, T. equi sequences from horses in Saint Kitts and Nevis clustered with sequences of genotype C. This study also identified two genotypes of T. equi in the equine population of Brazil. All of the T. equi and B. caballi sequences obtained from horses in Trinidad belong to genotype A and were similar to T. equi and B. caballi sequences of the same genotype that were submitted to GenBank™ databases. Countries in close proximity to Trinidad have T. equi sequences belonging to genotype C; therefore, movement of horses between these countries can introduce a new genotype of T. equi into the equid population of Trinidad.

Keywords

Babesia caballi Equine piroplasmosis Horses Phylogenetic analysis Theileria equi Trinidad 

Notes

Acknowledgements

The authors would like to thank the veterinarians, Drs. Michelle Branday, Clive Ali, Indira Pargass, Zinora Asgarali, Rossi Bridgelal, and Professor Asoke Basu who assisted with this study and all racehorse owners and managers of the farms involved who supplied data and monitored the mares and foals for this study.

Funding

This study was funded by The University of the West Indies Campus Research and Publication Fund (grant number 26600-457889).

Compliance with ethical standards

All procedures performed in this study involving animals were in accordance with the ethical standards of The University of the West Indies, St. Augustine at which the studies were conducted.

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Veterinary Medicine, Faculty of Medical SciencesThe University of the West IndiesSt AugustineTrinidad and Tobago
  2. 2.Department of Preclinical Sciences, School of Medicine, Faculty of Medical SciencesThe University of the West IndiesSt AugustineTrinidad and Tobago
  3. 3.Center for Infection and Immunity, Mailman School of Public HealthColumbia UniversityNew YorkUSA

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