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Outbreak of toxoplasmosis in a flock of domestic chickens (Gallus gallus domesticus) and guinea fowl (Numida meleagris)

  • Andréia VielmoEmail author
  • Hilda Fátima Jesus Pena
  • Welden Panziera
  • Ronaldo Michel Bianchi
  • Cíntia De Lorenzo
  • Solange Oliveira
  • Bruna Farias Alves
  • Solange Maria Gennari
  • Saulo Petinatti Pavarini
  • Claudio Severo Lombardo de Barros
  • David Driemeier
Protozoology - Original Paper
  • 26 Downloads

Abstract

Toxoplasmosis is a disease with a worldwide distribution that affects a wide variety of animal species, though with rare descriptions in chickens. We describe the clinical, epidemiological, pathological, and molecular aspects of a toxoplasmosis outbreak in domestic chickens and guinea fowl in southern Brazil. The flock was composed of 47 domestic chickens and 29 guinea fowl. Of these, 22 birds showed clinical signs of lethargy, anorexia, and neurological signs over a clinical course of 24–72 h, and 15 died. Epidemiological data were obtained through fieldwork performed at the chicken farm and necropsies of six birds. Gross lesions were absent at necropsy, and histopathological findings included inflammatory infiltrate of macrophages, lymphocytes, and plasma cells and necrosis in several tissues associated with intralesional Toxoplasma gondii. Immunohistochemistry for T. gondii was positive. Additionally, restriction fragment length polymorphism (RFLP) analysis with 11 markers (SAG1, SAG2 (5′3′SAG2 and alt. SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3) and microsatellite (MS) analysis with 15 markers (TUB2, W35, TgMA, B18, B17, M33, IV.1, XI.1, N60, N82, AA, N61, N83, M48, and M102) were performed. PCR-RFLP revealed T. gondii genotype ToxoDB-PCR-RFLP #280, and MS analysis also showed a unique genotype. This is the first description of this genotype in chickens and adds to the evidence suggesting considerable genotypic diversity of T. gondii in Brazil.

Keywords

Diseases of poultry Protozoal diseases Genotyping PCR-RFLP Microsatellite Pathology 

Notes

References

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Andréia Vielmo
    • 1
    Email author
  • Hilda Fátima Jesus Pena
    • 2
  • Welden Panziera
    • 1
  • Ronaldo Michel Bianchi
    • 1
  • Cíntia De Lorenzo
    • 1
  • Solange Oliveira
    • 2
  • Bruna Farias Alves
    • 2
  • Solange Maria Gennari
    • 2
  • Saulo Petinatti Pavarini
    • 1
  • Claudio Severo Lombardo de Barros
    • 3
  • David Driemeier
    • 1
  1. 1.Departamento de Patologia Clínica Veterinária, Setor de Patologia Veterinária, Faculdade de VeterináriaUniversidade Federal do Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  2. 2.Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e ZootecniaUniversidade de São Paulo (FMZ/USP)São PauloBrazil
  3. 3.Laboratório de Patologia Animal, Faculdade de Medicina Veterinária e ZootecniaUniversidade Federal de Mato Grosso do Sul (UFMS)Campo GrandeBrazil

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