Advertisement

Morphological and molecular characterization of Myxobolus dibombensis sp. n. (Myxozoa: Myxobolidae), a parasite of the African carp Labeobarbus batesii (Teleostei: Cyprinidae) from Dibombe River, Cameroon

  • Guy Benoît Lekeufack Folefack
  • Abdel-Azeem S. Abdel-Baki
  • Nelly Ornelle Onana Ateba
  • Abraham Fomena
  • Lamjed MansourEmail author
Fish Parasitology - Original Paper

Abstract

Myxobolus dibombensis sp. n. (Cnidaria: Myxosporea: Bivalvulida) is described from the fins of the African carp, Labeobarbus batesii, based on morphological and molecular data. Prevalence of infection was 51.9% (67/129). Ovoid to spherical cyst-like plasmodia were found in the intrasegmental region and among the fin rays. No pathological changes were found in the fish host tissue surrounding the cyst-like plasmodia. Mature myxospores were ovoid in frontal view and lenticular in lateral view, with slightly truncated anterior and rounded posterior ends. Myxospores measured 16.8 (15.8–18.0) μm long and 11.4 (10.0–13.0) μm wide. There was a triangular intercapsular appendix measuring 3.8 (2.6–4.5) μm long. Polar capsules were ovoid and slightly unequal in size, occupying approximately one-third of the myxospore length. The larger polar capsule measured 7 (6–8) μm long and 3.6 (3–4) μm wide, while the smaller one measured 5.8 (4.8–7.0) μm long and 3 (2–4) μm wide. The larger polar capsule contained nine to 11 filament coils, whereas the smaller one contained seven to nine coils. SSU rDNA gene sequence of M. dibombensis sp. n. did not match any sequences available in the GenBank. The similarity with available Myxobolus spp. sequences ranged from 65 to 81%. The novel species clustered with M. algonquinensis, which infects the cyprinid Luxilus cornutus from Canada.

Keywords

Myxobolus African carp SSU rDNA Phylogeny Fish diseases 

Notes

Funding information

The Dean of Scientific Research, King Saud University, funded the work through the research group project number RG-1435-023.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

References

  1. Ali MA, Al-Rasheid KA, Sakran T, Abdel-Baki AA, Abdel-Ghaffar FA (2002) Some species of the genus Myxobolus (Myxozoa: Myxosporea) infecting freshwater fish of the River Nile, Egypt, and the impact on their hosts. Parasitol Res 88:9–15Google Scholar
  2. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402CrossRefGoogle Scholar
  3. Andree KB, Székely C, Molnár K, Gresoviac SJ, Hedrick RP (1999) Relationships among members of the genus Myxobolus (Myxozoa: Bilvalvidae) based on small subunit ribosomal DNA sequences. J Parasitol 85:68–74CrossRefGoogle Scholar
  4. Becerra J, Montes GS, Bexiga SRR, Junqueira LCU (1983) Structure of the tail fin in teleosts. Cell Tissue Res 230:127–137CrossRefGoogle Scholar
  5. Camus AC, Dill JA, Rosser TG, Pote LM, Griffin MJ (2017) Myxobolus axelrodi n. sp. (Myxosporea: Myxobolidae) a parasite infecting the brain and retinas of the cardinal tetra Paracheirodon axelrodi (Teleostei: Characidae). Parasitol Res 116:387–397CrossRefGoogle Scholar
  6. Dyková I, Lom J (1978) Histopathological changes in fish gills infected with Myxosporidian parasites of the genus Henneguya. J Fish Biol 12:197–202CrossRefGoogle Scholar
  7. Eszterbauer E (2004) Genetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity. Dis Aquat Org 58:35–40CrossRefGoogle Scholar
  8. Fiala I (2006) The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 36:1521–1534CrossRefGoogle Scholar
  9. Fomena A, Bouix G, Birgi E (1985) Contribution to the study of freshwater fishes myxosporidia of Cameroon. III. New species of the genus Myxobolus Bütschli, 1882. Bull Inst fond Afr noire 46:169–192Google Scholar
  10. Fomena A, Marques A, Bouix G, Njine T (1994) Myxobolus bilongi n.sp., Thelohanellus assambai n.sp. et Thelohanellus sanagaensis n.sp., Myxosporidies parasites de Labeo sp (Téléosteen Cyprinidae) du Bassin de la Sanaga au Cameroun (Afrique Centrale). Ann Fac Sci Cameroun 1:31–42Google Scholar
  11. Fomena A, Abakar-Ousman NP, Bouix G (2004) Description of three new species of Myxosporidia (Myxozoa: Myxosporea) parasites of Citharinus citharus (Geofroy Saint Hilaire, 1809) (Citharinidae) in Chad (Central Africa). Parasite 11:83–88CrossRefGoogle Scholar
  12. Froese R, Pauly D (eds) (2015) “Cyprinidae” in FishBase. World Wide Web electronic publication http://www.fishbase.org/Summary/FamilySummary.php?Family=Cyprinidae. Accessed 25 Sep 2017
  13. Halliday MM (1976) The biology of Myxosoma cerebralis: the causative organism of whirling disease of salmonids. J Fish Biol 9:339–357CrossRefGoogle Scholar
  14. Holzer AS, Sommerville C, Wootten R (2004) Molecular relationships and phylogeny in a community of myxosporeans and actinosporeans based on their 18S rDNA sequences. Int J Parasitol 34:1099–1111CrossRefGoogle Scholar
  15. Karlsbakk E, Kristmundsson Á, Albano M, Brown P, Freeman MA (2017) Redescription and phylogenetic position of Myxobolus aeglefini and Myxobolus platessae n. comb. (Myxosporea), parasites in the cartilage of some North Atlantic marine fishes, with notes on the phylogeny and classification of the Platysporina. Parasitol Int 66:952–959CrossRefGoogle Scholar
  16. Kato E, Kasai A, Tomochi H, Li YC, Sato H (2017) Four Myxobolus spp. (Myxosporea:Bivalvulida) from the gill lamellae of common carp (Cyprinus carpio) and Japanese silver crucian carp (Carassius langsdorfii) in the western part of Japan, with the description of three new species (M. tanakai n. sp., M. paratoyamai n. sp., and M. ginbuna n. sp.). Parasitol Res 116:2427–2441CrossRefGoogle Scholar
  17. Kent ML, Andree KB, Bartholomew JL, El-Matbouli M, Desser SS, Devlin RH, Feist SW, Hedrick RP, Hoffmann RW, Khattra J, Hallett SL, Lester RJ, Longshaw M, Palenzeula O, Siddall ME, Xiao C (2001) Recent advances in our knowledge of the Myxozoa. J Eukaryot Microbiol 48:395–413CrossRefGoogle Scholar
  18. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  19. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Thompson JD (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948CrossRefGoogle Scholar
  20. Lekeufack FGB, Defoueng NAS, Fomena A (2017) Three new species of Myxobolus (Myxosporea: Myxobolidae), parasites of Barbus callipterus Boulenger, 1907 in Cameroon. Asian J Biol Sci 10:110–120CrossRefGoogle Scholar
  21. Liu XH, Batueva MD, Zhao YL, Zhang JY, Zhang QQ, Li TT, Li AH (2016) Morphological and molecular characterization of Myxobolus pronini n. sp. (Myxozoa: Myxobolidae) from the abdominal cavity and visceral serous membranes of the gibel carp Carassius auratus gibelio (Bloch) in Russia and China. Parasit Vectors 9:562CrossRefGoogle Scholar
  22. Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156CrossRefGoogle Scholar
  23. Lom J, Dyková I (2006) Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 53:1–36CrossRefGoogle Scholar
  24. Mansour L, Thabet A, Chourabi K, Harrath AH, Gtari M, Al Omar SY, Hassine OKB (2013) Kudoa azevedoi n. sp. (Myxozoa, Multivalvulida) from the oocytes of the Atlantic horse mackerel Trachurus trachurus (Perciformes, Carangidae) in Tunisian coasts. Parasitol Res 112:1737–1747CrossRefGoogle Scholar
  25. Moelants T (2010) Labeobarbus batesii. The IUCN red list of threatened species 2010: e.T182331A7861788. https://doi.org/10.2305/IUCN.UK.2010.RLTS.T182331A7861788.en. Accessed 27 Sep 2017
  26. Molnár K (2002) Site preference of myxosporean spp. on the fins of some Hungarian fish species. Dis Aquat Org 52:123–128CrossRefGoogle Scholar
  27. Molnár K, Székely C (2003) Infection in the fins of the goldfish Carassius auratus caused by Myxobolus diversus (Myxosporea). Folia Parasitol 50:31–36CrossRefGoogle Scholar
  28. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542CrossRefGoogle Scholar
  29. Sakiti N, Blanc E, Marques A, Bouix G (1991) Myxosporidia (Myxozoa, Myxosporea) of the genus Myxobolus Bütschli, 1882 parasites of cichlid fish from Lake Nokoué in Benin (West Africa). J Afr Zool 105:173–186Google Scholar
  30. Sanaullah M, Ahmed AT (1980) Gill myxoboliasis of major carps in Bangla­desh. J Fish Dis 3:349–354CrossRefGoogle Scholar
  31. Seenappa D, Manohar L (1981) Five new species of Myxobolus (Myxosporea: Protozoa), parasitic in Cirrhina mrigala (Hamilton) and Labeo rohita (Hamilton), with a note on a new host record for M. curmucae Seenappa & Manohar, 1980. J Protozool 28:358–360CrossRefGoogle Scholar
  32. Sitjà-Bobadilla A, Estensoro I, Pérez-Sánchez J (2016) Immunity to gastrointestinal microparasites of fish. Dev Comp Immunol 64:187–201CrossRefGoogle Scholar
  33. Stiassny MLG, Teugels GG, Hopkins CD (2007) Freshwater and brackish fish from lower Guinea, Western Central Africa. In: Hopkins (ed) Tropical Fauna and Flora Collection, IRD. Institute of Research for Development, Paris, p 797Google Scholar
  34. Tiogué CT, Tomedi MTE, Tchoumboué J (2013) Reproductive strategy of Labeobarbus batesii (Boulenger, 1903) (Teleostei: Cyprinidae) in the Mbô Floodplain Rivers of Cameroon. Int J Zool 2013:1–8CrossRefGoogle Scholar
  35. Vivien J (2012) Guide to mammals and fish in Cameroon. Ediprint, Saint BerthevinGoogle Scholar
  36. Whipps CM, Adlard RD, Bryant MS, Kent ML (2003) Two unusual myxozoans, Kudoa quadricornis n. sp. (Multivalvulida) from the muscle of goldspotted trevally (Carangoides fulvoguttatus) and Kudoa permulticapsula n. sp. (Multivalvulida) from the muscle of Spanish mackerel (Scomberomorus commerson) from the Great Barrier Reef, Australia. J Parasitol 89:168–173CrossRefGoogle Scholar
  37. Xiao C, Desser SS (1997) Sphaerospora ovophila n. sp. and Myxobolus algonquinensis n. sp. (Myxozoa, Myxosporea), ovarian parasites of fish from Algonquin Park, Ontario, Canada. J Eukaryot Microbiol 44:157–161CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Guy Benoît Lekeufack Folefack
    • 1
  • Abdel-Azeem S. Abdel-Baki
    • 2
    • 3
  • Nelly Ornelle Onana Ateba
    • 1
  • Abraham Fomena
    • 1
  • Lamjed Mansour
    • 2
    • 4
    Email author
  1. 1.Laboratory of Parasitology and Ecology, Department of Animal Biology and PhysiologyUniversity of Yaounde IYaoundeCameroon
  2. 2.Department of Zoology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  3. 3.Zoology Department, Faculty of ScienceBeni-Suef UniversityBeni-SuefEgypt
  4. 4.Laboratoire de Biodiversité, Parasitologie et Ecologie des Écosystèmes Aquatiques, Département de Biologie, Faculté des Sciences de TunisUniversité de Tunis El ManarTunisTunisia

Personalised recommendations