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Effect of regulatory T cells on the efficacy of the fatty acid-binding protein vaccine against Schistosoma japonicum

  • Chun-lian Tang
  • Rong-hui Zhang
  • Zhi-ming Liu
  • Huang JinEmail author
  • Long HeEmail author
Immunology and Host-Parasite Interactions - Original Paper
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Abstract

Schistosomiasis is one of the most devastating parasitic diseases, making it imperative to develop efficient vaccines to control the causative flatworms called schistosomes. Regulatory T cells (Tregs) and the Th1 immune response have been implicated in the effectiveness of vaccines to control schistosomiasis, but the mechanisms underlying their effects are unclear. In this study, we evaluated the role of Tregs on the efficacy of the 14 kDa FABP (fatty acid-binding protein) vaccine against Schistosoma japonicum. BALB/c female mice were randomly divided into five groups: an uninfected group, infected control group, anti-CD25 monoclonal antibody (anti-CD25 mAb) group, FABP group, and combined anti-CD25 mAb and FABP group. Compared with FABP alone, a combined treatment with FABP and anti-CD25 mAb increased the rate of S. japonicum inhibition in mice from 30.3 to 56.08% and decreased the number of eggs per gram of liver. Compared with that of the infected control group, the percentage of Tregs in the spleen decreased significantly after single or combined treatment with FABP and anti-CD25 mAb, while it increased gradually in the anti-CD25 mAb group. Further, the secretion of Th1 cytokines, IFN-γ, and IL-2 increased in splenocytes in the anti-CD25 mAb group. Our results indicate that anti-CD25 mAb partially blocks Tregs and concomitantly enhances the Th1 type immune response, thereby enhancing the protective effect of the FABP vaccine.

Keywords

Schistosoma japonicum Fatty acid-binding protein Regulatory T cells Anti-CD25 mAb Cytokines 

Notes

Funding

This research was funded by the Scientific Research Subject of the Health and Family Planning Commission of Wuhan Municipality (no. WX17A08; no. WX16B14; no. WX17Q17).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Wuchang Hospital affiliated to Wuhan University of Science and TechnologyWuhanChina
  2. 2.Department of Clinical Laboratory, Wuhan Fourth Hospital; Pu’ai hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  3. 3.Department of Clinical Laboratory, Shanghai East Hospital; School of MedicineTongji UniversityShanghaiChina

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