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Parasitology Research

, Volume 118, Issue 2, pp 421–432 | Cite as

Description of two new species of allocreadiid trematodes (Digenea: Allocreadiidae) in middle American freshwater fishes using an integrative taxonomy approach

  • David Iván Hernández-Mena
  • Carlos Daniel Pinacho-Pinacho
  • Martín García-Varela
  • Berenit Mendoza-Garfias
  • Gerardo Pérez-Ponce de LeónEmail author
Fish Parasitology - Original Paper
  • 92 Downloads

Abstract

Integrative taxonomy uses several sources of information to establish more robust species delimitation criteria. In this study, we followed that approach to describe two new species of allocreadiid trematodes from freshwater fish distributed in locations across Middle America. The new species were first recognized by using sequences of the domains D1–D3 of the ribosomal 28S rRNA gene and then, morphological data (light and scanning electron microscopy (SEM)), host association, and geographical distribution were considered as additional sources of information to complement the species delimitation and description. Auriculostoma tica n. sp. was found in the intestine of Gymnotus maculosus Albert and Miller in Costa Rica, while Wallinia anindoi n. sp. was found in the intestine of Astyanax aeneus (Günther) in Oaxaca and Chiapas, Mexico, as well as in a location of Guatemala. A phylogenetic analysis combining newly generated sequences of the two new species along with those available for other allocreadiids in GenBank, revealed that isolates of each species are reciprocally monophyletic, and also their interrelationships: Au. tica n. sp. is a sister taxon of Auriculostoma totonacapanensis Razo-Mendivil et al., 2014, and W. anindoi n. sp. is a sister taxon of Wallinia brasiliensis Dias, et al., 2018. Genetic distances for the 28S rRNA gene were estimated among the American species of allocreadiids and further supported the validity of the new species.

Keywords

Middle-America 28S rDNA Molecular phylogeny Auriculostoma Wallinia 

Notes

Acknowledgments

This study was part of the requirements of DIHM to obtain a PhD degree in the Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México. The authors thank Arturo Angulo, Universidad de Costa Rica, for his support to sample fish in Costa Rica under his collecting permit issued by Costa Rican government. We thank Luis García for providing specimens from the Colección Nacional de Helmintos for comparison, Leopoldo Andrade for his help during field work, Patricia Ornelas for her help in the collection and identification of fish in Mexico, and Laura Márquez for processing DNA samples for the automated sequencer.

Funding

This project was funded by the Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT-UNAM) IN202617 to GPPL, and PAPIIT-UNAM IN206716 to M.G.V. DIHM thanks the Consejo Nacional de Ciencia y Tecnología (CONACyT) for student grant 245193.

Compliance with ethical standards

All applicable international, national, and/or institutional guidelines for the use and care of animals were followed.

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Departamento de Zoología, Instituto de BiologíaUniversidad Nacional Autónoma de MéxicoMexicoMexico
  2. 2.Posgrado en Ciencias BiológicasUniversidad Nacional Autónoma de MéxicoMexicoMexico
  3. 3.Investigador Cátedras CONACyTInstituto de Ecología, A.C., Red de Estudios Moleculares AvanzadosVeracruzMexico

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