Biological evaluation of mimetic peptides as active molecules for a new and simple skin test in an animal model
A skin test is a widely used tool in diagnostic evaluations to investigate cutaneous leishmaniases (CL). The actual antigen (Montenegro skin test [MST] antigen) presents some difficulties that pertain to its manufacturing and validation. To contribute to overcoming this problem, we propose the application of new-generation molecules that are based on skin antigen tests. These antigens were obtained through biotechnology pathways by manufacturing synthetic mimetic peptides. Three peptides, which were selected by phage display, were tested as skin test antigens in an animal model (Cavia porcellus) that was immunized with Leishmania amazonensis or Leishmania braziliensis. The peptide antigens, individually (PA1, PA2, PA3) or in a mix (PAMix), promoted induration reactions at 48 and 72 h after the test was performed. The indurations varied from 0.5 to 0.7 cm. In the animals immunized with L. amazonensis, the PA3 antigen showed better results than the standard MST antigen. In animals immunized with L. braziliensis, two peptide antigens (PA2 and PAMix) promoted induration reactions for a longer period of time than the standard MST antigen. These results validate our hypothesis that peptides could be used as antigens in skin tests and may replace the current antigen for CL diagnosis.
KeywordsPhage display Synthetic peptides Diagnostic Neglected disease
We thank the Centro de Produção e Pesquisa de Imunobiológicos (CPPI) of SESA, Paraná State, for technical support and for providing the MST antigen for this study.
This study was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; Grant Nos. 307387/2011-9 and 480292/2012-4), Fundação Araucária (Grant No. 122/2010 [Protocol 17401]), the Programa Nacional de Pós-Doutorado-Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PNPD-CAPES; Protocol 2847/2011), and scholarships granted to the two first author.
Compliance with ethical standards
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The study was approved by the Research Ethics Committee of the Federal University of Parana (Process no. 101328/2015-69). This article does not contain any studies that were performed by any of the authors with human subjects.
Conflict of interest
The authors declare that they have no competing interests.
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