Parasitology Research

, Volume 117, Issue 11, pp 3391–3397 | Cite as

First report on Protostrongylus cuniculorum (Nematoda: Protostrongylidae) in the European brown hare (Lepus europaeus Pallas, 1778 L.) from Bulgaria

  • Mariana Panayotova-PenchevaEmail author
  • Vassilena Dakova
  • Anetka Trifonova
Original Paper


A nematode species from the Protostrongylidae family, unknown in the parasite fauna of Bulgaria until now, was found in the bronchi of a European brown hare (Lepus europaeus Pallas, 1778 L.) from a southwestern region of the country. At first the systematic identification of the found specimens was attributed to two possibilities—Protostrongylus terminalis Passerini (1884) Schulz, Orlow & Kutass, 1933 and Protostrongylus cuniculorum (Joyeux & Gaud, 1946) Schulz & Kadenazii, 1949. The autonomy of these two species was discussed based on data available in the literature. Morphological and morphometric data about the male and female specimens are provided in the present materials. After comparing these data with the ones available in the literature, the helminths were related to the species P. cuniculorum. P. cuniculorum is reported for the first time as part of the helminth fauna of the European brown hare from Southeastern Europe and Bulgaria in particular.


European brown hare Lepus europaeus L. Protostrongylus cuniculorum Protostrongylus terminalis Morphometric features 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Allgower R (1992) Der Parasitenbefall von Feldhasen aus der Oberrheinebene und seine intraspezifische Bedeutung. Z Jagdwiss 38:116–127Google Scholar
  2. Babos S (1961) Zur Kenntnis der Protostrongylosen der Leporiden, unter besonderer Berücksichtigung der in Ungarn vorkommenden Protostrongylus. Ancn Helminthol 3:13–37Google Scholar
  3. Barbiera A (1960) Su alcuni casi di strongilosi polmonare della lepre. Indagini parassitolo-giche. Ann Facolta di Medicina Veterinaria 13:19–25Google Scholar
  4. Blasco S, Torres J, Feliu C, Casanova JC, Miquel J, Moreno S (1996) The helminthfauna of Oryctolagus cuniculus (Linnaeus, 1758) in the Iberian Peninsula. Faunistic and ecological considerations. Parasite 4:327–333CrossRefGoogle Scholar
  5. Boev SN (1950) Contribution to the classification of the lung nematodes of the genus Protostrongylus Kamensky, 1905. Trudy Gel'mintologicheskoi Laboratorii AN SSSR 4:64–67 (in Russian)Google Scholar
  6. Boev SN (1975) Protostrongylidi. In: Rijikov K (ed) Osnovy nematodologii, XXV vol. Izdatelstvo Nauka, Moskva 264 pp (in Russian)Google Scholar
  7. Burgaz I (1970) A report on the presence of endoparasites of Lepus timidus L. and Lepus europaeus Pallas in Sweden. Nytt Magasin for Zoologi 18:100Google Scholar
  8. Chroust K, Vodnansky M, Pikula J (2012) Parasite load of European brown hares in Austria and the Czech Republic. Vet Med 57:551–558CrossRefGoogle Scholar
  9. Costantini R, Manfredi MT, Iori A, Pacetti A (1990) Protostrongylus pulmonalis from hares (Lepus europaeus) in Italy. Parassitologia 32:353–357PubMedGoogle Scholar
  10. Czaplinska D, Czaplinski B, Rutkowska M, Zebrowska D (1965) Studies on the European hare. IX. Helminth fauna in the annual cycle. Acta Theriologica Bialowieza 10:55–78CrossRefGoogle Scholar
  11. Fataliyev GG (2013) Helminthofauna hare Azerbaijan and their formation. Vesnik Zaporizkogo Nacionalnogo Universitetu 3:53–59Google Scholar
  12. Forstner MJ, Ilg V (1982) Untersuchungen über die endoparasiten des Feldhasen (Lepus europaeus) und Versuche zu ihrer Bekämpfung. Z Jagdwiss 28:169–177Google Scholar
  13. Frolich K, Wisser J, Schmuser H, Fehlberg U, Neubauer H, Grunow R, Nikolaou K, Priemer J, Thiede S, Streich WJ, Speck S (2003) Epizootologic and ecologic investigations of European brown hare (Lepus europaeus) in selected populations from Schleswig-Holstein, Germany. J Wildl Dis 39:751–761CrossRefGoogle Scholar
  14. Genov Т (1970) Edin nov i opasen parasit po zaitsite u nas. Priroda 19:65–68 (in Bulgarian)Google Scholar
  15. Gottschalk C (1973) Endoparasiten der feldhasen in ihrer rolle für die niederwildjagd Ostthuringens. Angew Parasitol 14:44–54PubMedGoogle Scholar
  16. Ilic T, Petrovic T, Dimitrijevic S (2014) Parasitic infections of wild rabbits and hares. Vet Glasnik 68:241–250CrossRefGoogle Scholar
  17. Joyeux S, Gaud J (1946) Recherches helminthologiques Marocaines. Arch de l’Inst Pasteur du Maroc 3:383–461Google Scholar
  18. Kontrimavichus VL, Deliamure SL, Boev SN (1976) Metastrongyloidei domashnih i dikih jivotnih. In: Rijikov K (ed) Osnovy nematodologii, XXVI vol. Izdatelstvo Nauka, Moskva 237 pp (in Russian)Google Scholar
  19. Kornas S, Wierzbowska IA, Wajdzik M, Kowal J, Basiaga M, Nosal P (2014) Endoparasites of European brown hare (Lepus europaeus) from southern Poland based on necropsy. Ann Anim Sci 14:297–306CrossRefGoogle Scholar
  20. Kutzer E, Frey H (1976) Die parasiten der feldhasen (Lepus europaeus) in Österreich. Berl Munch Tierarztl Wochenschr 89:480–483PubMedGoogle Scholar
  21. Lesage C, Jouet D, Patrelle C, Guitton JS, Decors A, Ferte H (2014) Protostrongylus pulmonalis (Frölich, 1802) and P. oryctolagi Baboš, 1955 (Nematoda: Protostrongylidae), parasites of the lungs of European hare (Lepus europaeus L.) in France: morphological and molecular approaches. Parasitol Res 113:2103–2111CrossRefGoogle Scholar
  22. Merkusheva IV (1960) The helminth fauna of Lepus europaeus and L. timidus in Byelorussian S.S.R. Trudy Nauchno-Issledovatelskogo Veterinarnogo Instituta 1:211–216 (in Russian)Google Scholar
  23. Movsesyan SO, Panayotova-Pencheva MS, Demiaszkiewiz AW, Voronin MV (2016) Host-based formation of fauna of lung helminths, its biological and taxonomic classification. Russ J Parasitol 37:345–369Google Scholar
  24. Nickel S, Gottwald A (1979) Parasites of the GDR. 3. Endoparasites of the hare (Lepus europaeus). Angew Parasitol 20:57–62PubMedGoogle Scholar
  25. Panayotova-Pencheva M (2011) Species composition and morphology of protostrongylids (Nematoda: Protostrongylidae) in ruminants from Bulgaria. Parasitol Res 109:1015–1020CrossRefGoogle Scholar
  26. Panayotova-Pencheva M, Alexandrov M (2008) Morphometric characteristics of first stage Elaphostrongylus cervi (Nematoda: Protostrongylidae) larvae from Bulgaria. Eur J Wildl Res 54:771–774CrossRefGoogle Scholar
  27. Panayotova-Pencheva M, Trifonova A, Mirchev R, Zhelev Ch, Salkova D (2014) Actual data on Protostrongylus tauricus (Nematoda: Protostrongylidae) in materials from Bulgaria. In: Proceedings of the international scientific conference: "20 years Faculty of Veterinary Medicine at the University of Forestry", Izdatelska kashta pri LTU, Sofia, pp 238–244 (in Bulgarian)Google Scholar
  28. Passerini N (1884) Sulla Filaria terminalis auctor. Atti Soc Ital Sci Nat 27:42–63Google Scholar
  29. Rodonaia T (1967) On the study of helminthes of Lepus europaeus in Georgia. Gel’mintofauna Zhivotnykh i Rasteniĭ v Gruzii 98–104 (in Georgian)Google Scholar
  30. Shults RS, Kadenazii АN (1949) Filogeneticheskie svyazi legochnih nematode grizunov i parnokopitnih. Dokladi AN SSSR 69:707–709Google Scholar
  31. Sitko M, Mituch J, Konrad V, Spenik M (1970) Occurrence of Protostrongylus infection in hares and the pathological changes caused by it. Veterinarsky Casopis 13:163–167Google Scholar
  32. Soveri T, Valtonern M (1983) Endoparasites of hares (Lepus timidus L. and L. europaeus Pallas) in Finland. J Wildl Dis 19:337–341CrossRefGoogle Scholar
  33. Yanchev Y (1970) Untersuchungen uber die helminthenfauna des feldhasen (Lepus europaeus Pall.) in Bulgarien. III. Materialen uber die helminthenfauna des feldhasen (Lepus europaeus Pall.) in Sudwestbulgarien. Bulletin de l’Institute de Zoologie et Musee 32:107–115 (in Bulgarian)Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mariana Panayotova-Pencheva
    • 1
    Email author
  • Vassilena Dakova
    • 1
  • Anetka Trifonova
    • 2
  1. 1.Institute of Experimental Morphology, Pathology and Anthropology with MuseumBulgarian Academy of SciencesSofiaBulgaria
  2. 2.South-West State EnterpriseSofiaBulgaria

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