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Parasitology Research

, Volume 117, Issue 10, pp 3145–3156 | Cite as

Morphological and molecular characterization of Chloromyxum argusi n. sp. (Myxosporea) infecting the urinary bladder of Scatophagus argus Linnaeus 1766 (Scatophagidae) from the southwest coast of India

  • Archana Chandran
  • P. U. Zacharia
  • T. V. Sathianandan
  • P. Shamal
  • C. P. Binesh
  • Pinky Kaur
  • N. K. Sanil
Original Paper

Abstract

The present paper describes a new species of Chloromyxum infecting the urinary bladder of the estuarine fish, Scatophagus argus, from the southwest coast of India. The parasite exhibited an overall prevalence of 41.93%; the prevalence is influenced by host size and seasons. Mature spores are subspherical, measure 9.40 ± 0.66 by 9.32 ± 0.87 μm, and are characterized by the presence of sutural and extra-sutural ridges, binucleated sporoplasm, and a pair of caudal extensions. Four pyriform, unequal polar capsules with raised polar filament discharge pores and ribbon-like polar filaments are present. Polar filament coils numbered four to five in large polar capsules and three in small polar capsules. Pansporoblast is irregular with granulated cytoplasm and has fine villosites on its surface. Plasmodia are spherical/irregular with monosporic and polysporic forms. In molecular and phylogenetic analysis, the myxosporean stands out with a high bootstrap value and was positioned as a sister branch of Chloromyxum kurisi. In view of the morphologic, morphometric, and molecular differences with the existing species of Chloromyxum, and considering the differences in hosts and geographic locations, the present species is treated as new and the name Chloromyxum argusi n. sp. is proposed.

Keywords

Chloromyxum argusi n. sp. Scatophagus argus Myxosporeans Spores Extra-sutural ridges Caudal extension Urinary system Molecular phylogeny 

Notes

Acknowledgements

The authors wish to thank the Director, Central Marine Fisheries Research Institute, Cochin, and the Indian Council of Agricultural Research, New Delhi, for providing necessary facilities for undertaking this work. The authors also wish to thank Mr. K.M. David for the line drawings.

Financial support

The present study was supported by the Council of Scientific & Industrial Research, New Delhi, through a Senior Research Fellowship for the first author (No. 09/1135(0001)/2015-EMR-I).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Archana Chandran
    • 1
  • P. U. Zacharia
    • 1
  • T. V. Sathianandan
    • 1
  • P. Shamal
    • 1
  • C. P. Binesh
    • 1
  • Pinky Kaur
    • 1
  • N. K. Sanil
    • 1
  1. 1.Central Marine Fisheries Research InstituteKochiIndia

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