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Parasitology Research

, Volume 117, Issue 10, pp 3137–3143 | Cite as

A novel molecular and chromosomal lineage of the anthropophilic Simulium (Simulium) rufibasis subgroup (Diptera: Simuliidae) in Taiwan

  • Van Lun Low
  • Hiroyuki Takaoka
  • Peter H. Adler
  • Tiong Kai Tan
  • Francis Cheng-Hsuan Weng
  • Cheng-Yu Chen
  • Yvonne Ai Lian Lim
  • Zubaidah Ya’cob
  • Chee Dhang Chen
  • Mohd Sofian-Azirun
  • Daryi Wang
Original Paper

Abstract

The Simulium rufibasis subgroup is one of three subgroups of the Simulium (Simulium) tuberosum species-group; it is characterized by a pair of clustered stout hairs on the ventral surface of female abdominal segment 7. A member of the S. rufibasis subgroup in Taiwan was investigated morphologically and genetically using the universal cytochrome c oxidase subunit I (COI) barcoding gene and polytene chromosomal banding pattern. The Taiwanese material is morphologically similar to S. rosliramlii Takaoka & Chen from Vietnam and represents the second species of the S. rufibasis subgroup known from Taiwan. It also represents a novel molecular lineage that is distinct from three other primary lineages identified as S. doipuiense, S. doipuiense/S. rufibasis, and S. weji previously reported from Thailand. The mitochondrial evidence for a distinct lineage in Taiwan is supported by chromosomal analysis, which revealed unique sex chromosomes. For nomenclatural stability, we associate the name S. arisanum Shiraki with the Taiwanese entity. Originally described from females from Taiwan, S. arisanum until now has remained an enigmatic species.

Keywords

Black fly COI gene Species complex Polytene chromosomes Taiwan 

Notes

Funding information

This work was supported by research grants from University of Malaya (RP021C-16SUS and RU005-201). For PHA, this is Technical Contribution of the Clemson University Experiment Station (6658) and is based on work supported in part by NIFA/USDA under project number SC-1700527.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Van Lun Low
    • 1
  • Hiroyuki Takaoka
    • 1
  • Peter H. Adler
    • 2
  • Tiong Kai Tan
    • 3
  • Francis Cheng-Hsuan Weng
    • 4
  • Cheng-Yu Chen
    • 4
  • Yvonne Ai Lian Lim
    • 3
  • Zubaidah Ya’cob
    • 1
  • Chee Dhang Chen
    • 5
  • Mohd Sofian-Azirun
    • 5
  • Daryi Wang
    • 4
  1. 1.Tropical Infectious Diseases Research and Education Centre (TIDREC)University of MalayaKuala LumpurMalaysia
  2. 2.Department of Plant and Environmental SciencesClemson UniversityClemsonUSA
  3. 3.Department of Parasitology, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia
  4. 4.Biodiversity Research CenterAcademia SinicaTaipeiTaiwan
  5. 5.Institute of Biological Sciences, Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia

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