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Parasitology Research

, Volume 117, Issue 10, pp 3315–3318 | Cite as

Molecular verification of transplacental transmission of Theileria lestoquardi in goat

  • Bijan Esmaeilnejad
  • Mousa Tavassoli
  • Awat Samiei
  • Nasser Hajipour
Short Communication

Abstract

Ovine and caprine malignant theileriosis (OCMT), a critical condition in small ruminant production, causes lethal infections. In September 2016, a total number of 400 goats of Marghoz breed (the largest population of goat breed in Iran), in northwest of Iran, were examined for approximately 25 pre-partum abortions and 7 goats’ mortality for a period of 3 days. A dead goat and her aborted fetus were brought into the Urmia Veterinary Hospital for further diagnostic investigations. The microbiological assessments including direct microscopical examination of the vaginal discharges and placentome with respect to the differential staining (Gram’s staining method), conventional pure culturing, fetal abomasal contents, and the liver were negative. Microscopic examinations of blood smears of the goat and the fetus revealed characteristic of Theileria spp. piroplasms and impression smear samples from goat liver and fetal spleen were positive for Theileria Koch blue bodies. DNA analysis was performed using PCR technique and specific primers derived from the nucleotide sequences of 18S rRNA gene of T. lestoquardi; following extraction from blood samples, placentome, goat liver, and spleen of the fetus. The amplified DNA was sequenced afterwards and the corresponding sequence was registered under GenBank accession number MG208059. The sequence alignment showed that the products of PCR had a homology of 99% to known T. lestoquardi sequence registered under accession numbers of KY352037.1, KC778786.1, and JQ917458.1 in the GenBank. To our knowledge, this is a report demonstrating molecular verification of T. lestoquardi transplacental transmission in a neonatal kid of Marghoz breed of goats, its feasible role in induction of perinatal deaths and abortion in goat flocks.

Keywords

Theileria lestoquardi Transplacental transmission Goat Abortion PCR 

Notes

Acknowledgments

The authors would like to thank the staff at Parasitology Division of Faculty of Veterinary Medicine, Urmia University, Iran for their helps.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Bijan Esmaeilnejad
    • 1
  • Mousa Tavassoli
    • 1
  • Awat Samiei
    • 1
  • Nasser Hajipour
    • 2
  1. 1.Department of Pathobiology, Faculty of Veterinary MedicineUrmia UniversityUrmiaIran
  2. 2.Department of Pathobiology, Faculty of Veterinary MedicineUniversity of TabrizTabrizIran

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