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Evaluation of parasite and host genetics in two generations of a family with Chagas disease

Abstract

Chagas disease, caused by the protozoan Trypanosoma cruzi, is considered to be a multifactorial disease associated with host and parasite genetics, which influence clinical aspects of the disease and other host conditions. In order to understand better the evolution of the disease, this study intended to evaluation of parasite and host genetics in two generations of a family with Chagas disease from the Alto Paranaiba region, Minas Gerais, Brazil, comprising a mother and her five daughters. Several features were evaluated, including the characterization of T. cruzi directly from the blood of patients, host polymorphisms of genes related to cardiomyopathy (TNF, WISP1, CCR5, and TGF-β1) and clinical aspects of the patients. To verify the intraspecific variability of the parasite, the characterization was done directly from human blood using the PCR-LSSP technique and analyzed based on Dice coefficient and unweighted pair group analysis (UPGMA). The host polymorphism was evaluated by PCR-RFLP. The global results showed low variability of the parasites characterized from blood of patients, through Shannon index (0.492) and mean heterozygosity value per locus (0.322). All six patients presented the same genetic polymorphism profile for TNF, WISP1, and TGF-β1, and only one patient was homozygous to CCR5, which suggests that there is no association between the clinical aspects of the patients and their genetic profiles. In conclusion, the findings confirm that the understanding of the clinical evolution of Chagas disease goes beyond the genetic aspects of the parasite and the host.

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Acknowledgments

The authors thank the subjects whose participation made this study possible. We would also like to thank FAPEMIG (CBB-APQ-01497-14), CNPq (CNPq-441836/2014-3), CAPES, and Universidade Federal de Ouro Preto (UFOP). Authors are grateful to the assistance provided by Levi Soares Reis from the “Laboratório de Imunopatologia/NUPEB/UFOP,” Nayara Toledo from “Laboratório de Bioquímica/NUPEB/UFOP,” and Profa. Claudia Rachel de Melo from “Centro Universitário de Patos de Minas” for their attention and support.

Author information

Correspondence to Glenda Nicioli da Silva.

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Competing interests

The authors declare that they have no competing interests.

Ethical approval

All patients consented to participate in the study by signing the informed consent form approved by the Ethics Committee on Human Research at the UFOP, protocol 1.328.756.

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Lima, A.P.B., de Oliveira, M.T., Silva, R.R. et al. Evaluation of parasite and host genetics in two generations of a family with Chagas disease. Parasitol Res 117, 3009–3013 (2018). https://doi.org/10.1007/s00436-018-5969-5

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Keywords

  • T. cruzi genetics
  • Host genetic polymorphism
  • Chagas disease evolution