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Molecular characterization of the Taenia solium Tso31 antigen and homologous of other Taenia species from Peru

  • Ana Vargas-CallaEmail author
  • Luis A. Gomez-Puerta
  • Maria T. Lopez
  • Hector H. Garcia
  • Armando E. Gonzalez
  • for the Cysticercosis Working Group in Peru
Helminthology - Short Communication
  • 18 Downloads

Abstract

Several studies have been performed to determine specific antigens for the diagnosis of tapeworms. One of these antigens is Tso31, which is used to differentiate Taenia solium and Taenia saginata in human feces. The aim of the present work was the molecular characterization of this protein in different tapeworm specimens collected in Peru: T. omisa (n = 6), T. hydatigena (n = 7), T. taeniaeformis (n = 4), T. pisiformes (n = 1), T. multiceps (n = 7), and T. solium (n = 10). Total DNA was extracted from each proglottid using a commercial DNA kit for tissue. A nested PCR was used to amplify a fragment of the previously described oncosphere-specific protein Tso31 gene. The nested PCR products were analyzed by 1.5% agarose gel electrophoresis and visualized after ethidium bromide staining. All nested PCR-positive products were sequenced and their sequences were compared. Of all the tapeworms analyzed, only T. solium and T. multiceps amplified the Tso31 gene. All sequences were identical for each species. Our T. solium Tso31 showed 100% similarity when compared with published GenBank sequences. The difference between T. solium and T. multiceps Tso31 samples was 8.1%. In conclusion, our results show that the tsol31 gene is not exclusive to T. solium.

Keywords

Taenia solium Tso31 gene Homologous Tapeworms 

Notes

Funding information

This work was partially supported by FIC/NIH Training Grant TW001140.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ana Vargas-Calla
    • 1
    Email author return OK on get
  • Luis A. Gomez-Puerta
    • 1
  • Maria T. Lopez
    • 1
  • Hector H. Garcia
    • 2
    • 3
  • Armando E. Gonzalez
    • 1
  • for the Cysticercosis Working Group in Peru
  1. 1.School of Veterinary MedicineUniversidad Nacional Mayor de San MarcosLimaPeru
  2. 2.Department of Microbiology and Center for Global HealthUniversidad Peruana Cayetano HerediaLimaPeru
  3. 3.Cysticercosis UnitInstituto Nacional de Ciencias NeurológicasLimaPeru

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