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Development and evaluation of a rapid diagnostic immunochromatographic device to detect antibodies in sera from intestinal capillariasis cases


Intestinal capillariasis, a fish-borne nematodiasis, is an important emerging zoonotic disease. Patients present clinical symptoms of borborygmus chronic diarrhea, intermittent abdominal pain, weight loss, and several degrees of painless lower-leg edema. Death may occur in cases of misdiagnosis and improper treatment. Diagnosis is difficult because of the atypical clinical symptoms and diagnostic confusion with diarrhea caused by gastrointestinal cancer, opportunistic infections in human immunodeficiency virus patients, and hyperthyroidism. In addition, parasite eggs are not always found in stool examination. Serology can provide a supportive diagnostic tool. We have produced a rapid and simple immunochromatographic test (ICT) kit for diagnosis of intestinal capillariasis by detection of diagnostic antibodies in human sera. Serum samples from healthy volunteers and patients with proven intestinal capillariasis and other parasitic diseases were evaluated with the Trichinella spiralis larval extract antigen absorbed ICT strips. The diagnostic sensitivity, specificity, and positive and negative predictive values were 100, 96.6, 90.2, and 100%, respectively. The ICT kit is simple and rapid to use and can supplement stool examination in clinical diagnosis of intestinal capillariasis. The test can be completed in 15 min without a need for any sophisticated instruments or reagents.

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We wish to acknowledge the support of the English Consultation Clinic at the Khon Kaen University Faculty of Medicine Research Affairs Division and the Khon Kaen University Publication Clinic at the Research and Technology Transfer Affairs Division for their assistance. This study was supported by a TRF Senior Research Scholar Grant, Thailand Research Fund grant number RTA5880001; Research Result Utilization, Faculty of Medicine, Khon Kaen University (grant number RU59302); Target Research Grant, Faculty of Medicine, Khon Kaen University (grant number TR57201) through WM and PM. RR was supported by the Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program (grant no. PHD/0053/2556). OS were supported by scholarship under the post-doctoral training program from Research Affairs and Graduate School, Khon Kaen University (grant no. 58101).

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Correspondence to Pewpan M. Intapan.

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The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Ethical approval

Informed consents were gotten from all human adult participants and from parents or legal guardians of minors. The study protocol was accepted by the Khon Kaen University Ethics Committee for Human Research (HE 591318).

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Intapan, P.M., Rodpai, R., Sanpool, O. et al. Development and evaluation of a rapid diagnostic immunochromatographic device to detect antibodies in sera from intestinal capillariasis cases. Parasitol Res 116, 2443–2447 (2017). https://doi.org/10.1007/s00436-017-5546-3

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  • Intestinal capillariasis
  • Capillaria philippinensis
  • Antibody detection
  • Immunochromatographic test