Human strongyloidiasis is a deleterious gastrointestinal disease mainly caused by Strongyloides stercoralis infection. Strongyloides stercoralis is a soil-transmitted helminthiasis that is distributed around the globe. Although definitive diagnosis is carried out through the detection of parasite objects in human stool samples, the development of reliable immunological assays is an important alternative approach for supportive diagnosis. We characterized the two sensitive and specific bands of S. stercoralis filariform larvae that reacted with human strongyloidiasis sera based on immunoblot analysis. Serum samples obtained from strongyloidiasis patients showed a sensitivity of 90 and 80 % at the approximate molecular mass of 26 and 29-kDa polypeptide bands, respectively. The reactive specificity of the 26-kDa band was 76.5 % while for the 29-kDa band was 92.2 %. Proteomic analysis identified the 26-kDa band protein was 14-3-3 protein zeta, while the 29-kDa band protein was ADP/ATP translocase 4. The results provided a basic framework for further studies regarding the potential of the S. stercoralis recombinant antigen to become a leading to diagnostic tool.
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We wish to acknowledge the support of the English Consultation Clinic at the Khon Kaen University Faculty of Medicine Research Affairs Division and the Khon Kaen University Publication Clinic at the Research and Technology Transfer Affairs Division for their assistance.
All procedures performed in studies involving human participants were in accordance with ethical standards of the Khon Kaen University Ethics Committee for Human Research (HE591192) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Conflict of interests
The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
This study was supported by a TRF Senior Research Scholar Grant, Thailand Research Fund grant number RTA5880001; the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, Thailand, through the Health Cluster (SHeP-GMS); the Faculty of Medicine, Khon Kaen University (grant no. TR57201) through WM and PM. RR was supported by the Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program (grant no. PHD/0053/2556). OS were supported by Post-Doctoral Training Program of Graduate School and Khon Kaen University (grant no. 58101).
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Rodpai, R., Intapan, P.M., Thanchomnang, T. et al. Strongyloides stercoralis diagnostic polypeptides for human strongyloidiasis and their proteomic analysis. Parasitol Res 115, 4007–4012 (2016). https://doi.org/10.1007/s00436-016-5170-7
- Strongyloides stercoralis
- Proteomic analysis
- Immunoblot analysis