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Morphological and molecular characterization of Henneguya bicaudi n. sp. (Myxosporea: Myxobolidae) infecting gills of Cirrhinus mrigala (Ham.) in Harike Wetland, Punjab (India)

Abstract

In the present study, Henneguya bicaudi n. sp. infecting gills of Cirrhinus mrigala (Ham.) an Indian major carp from Harike Wetland in Punjab, India, has been described on the basis of myxospore morphology and partial amplification of the SSU rRNA gene. The pseudocysts of H. bicaudi n. sp. were located within the gill filament, whitish with round to irregular outer margin and 0.5–0.7 mm in diameter, 3–4 pseudocysts each containing about 50–100 myxospores were detected per gill. Mature myxospores were oblongate, 21.0 × 6.2 μm had a caudal appendage bifurcating into two thin thread-like branches starting posterior to middle of its length. The two equal polar capsules were 3.14 × 2.02 μm. The present species was compared morphologically with 21 species of the genus Henneguya infecting fresh water fishes in India and related geographical area. H. bicaudi n. sp. differed in having a peculiar oblongate shape and size of the myxospore, equal polar capsules, and also by the location of the point of bifurcation of the caudal appendage. 18S rDNA sequences of H. bicaudi n. sp. obtained revealed 96 % homogeneity with H. pseudorhinogobi (Kageyama et al., Fish Sci 75:657–663, 2009) infecting gill filaments of Rhinogobius sp. (Rutter, PANS Philad 56-90, 1897) in Japan. The intensity of infection was determined by the gill pseudocyst index (GPI) based on the number of countable pseudocysts by stereo zoom trinocular microscopy per gill (on one side).

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Acknowledgments

Thanks are due to funding by the University Grants Commission (UGC) in the form of Major Research Project (MRP). The authors are grateful to Dr. Sukhbir Kaur, Professor in the Department of Zoology, Panjab University, Chandigarh for providing the infrastructure in her Laboratory, and special thanks are due to Dr. Jyoti Joshi, Department of Zoology, Panjab university, Chandigarh for guidance during the experimental work.

Compliance with ethical standards

The authors declare that they have no competing interests.

Financial support was given by the University Grants Commission(UGC) New Delhi in the form of Major Research Project (MRP).

State of welfare of animals

The fishes were collected in fresh form from the fisherman at the wetland site and also from the local fish market near the wetland site.

At the point of time when work began on the myxozoan parasites of freshwater fishes, we approached Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCEA), Ministry of Environment, Forest and Climate Change, and Government of India for ethical clearance. The committee informed that CPCEA instruction’s protocol for experimentation on fishes does not require CPCEA approval.

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Correspondence to Harpreet Kaur.

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Kaur, H., Attri, R. Morphological and molecular characterization of Henneguya bicaudi n. sp. (Myxosporea: Myxobolidae) infecting gills of Cirrhinus mrigala (Ham.) in Harike Wetland, Punjab (India). Parasitol Res 114, 4161–4167 (2015). https://doi.org/10.1007/s00436-015-4647-0

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Keywords

  • Henneguya
  • Cirrhinus mrigala
  • 18S rDNA
  • Phylogeny