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Prevention of Lactogenic Toxocara cati Infections in Kittens by Application of an Emodepside/Praziquantel Spot-on (Profender®) to the Pregnant Queen

Abstract

This study aimed to evaluate the efficacy of an emodepside 2.1 % (w/v)/praziquantel 8.6 % (w/v) topical solution (ProfenderR spot-on for cats) in the prevention of lactogenic Toxocara cati infections. A controlled test was performed with two groups of 8 cats with confirmed pregnancy. All cats were infected with daily doses of 2000 T. cati eggs for 10 consecutive days starting 50 days post conception to produce an acute infection. Treatment was performed 60 days post conception. Queens in the treatment group received the emodepside/praziquantel solution at the minimum therapeutic dose (3 mg/kg emodepside and 12 mg/kg praziquantel), while the control group was treated with a placebo spot-on. Efficacy was evaluated 56 days post partum by necropsy of one randomly selected kitten of each litter and comparison of the worm burdens between the study groups. Additionally the necropsy results were supported by quantification of worms expelled with the faeces after deworming of the remaining kittens and all queens. The treatment in late pregnancy resulted in an efficacy of 98.7 % (p < 0.0001). All necropsied control kittens were infected (geometric mean 30.6). Seven of 8 kittens from treated mothers were free of T. cati (geometric mean 0.4). Worm counts after deworming reflected the results obtained at necropsy. No side effects of the treatment were observed. It is concluded that treatment with an emodepside/praziquantel spot-on solution during late pregnancy effectively prevents lactogenic transmission of T. cati to the offspring. The study design facilitated the generation of reliable data, while at the same time a minimum number of animals was sacrificed.

References

  1. Barth D, Rehbein S, Reid JF, Barrick RA (1996) Efficacy of an in-feed formulation of ivermectin against adult worms and somatic larvae of Strongyloides ransomi. Vet Parasitol 65:89–97

  2. Barutzki D, Schaper R (2013) Age-dependant prevalence of endoparasites in young dogs and cats up to one year of age. Parasitol Res 112(Suppl 1):119–131

  3. Becker AC, Rohen M, Epe C, Schnieder T (2012) Prevalence of endoparasites in stray and fostered dogs and cats in Northern Germany. Parasitol Res 111:849–857

  4. Burke TM, Roberson EL (1985a) Prenatal and lactational transmission of Toxocara canis and Ancylostoma caninum: experimental infection of the bitch before pregnancy. Int J Parasitol 15:71–75

  5. Burke TM, Roberson EL (1985b) Prenatal and lactational transmission of Toxocara canis and Ancylostoma caninum: experimental infection of the bitch at midpregnancy and at parturition. Int J Parasitol 15:485–490

  6. Coati N, Schnieder T, Epe C (2004) Vertical transmission of Toxocara cati Schrank 1788 (Anisakidae) in the cat. Parasitol Res 92:142–146

  7. Conn DB, Etges FJ (1983) Maternal transmission of asexually proliferative Mesocestoides corti tetrathyridia (Cestoda) in mice. J Parasitol 69:922–925

  8. Drag MD, Green SE, Howser RA, Wallace DH, Cox JL, Barrick RA (1998) Efficacy of an in-feed formulation of ivermectin against somatic larvae of Strongyloides ransomi in pregnant swine. Am J Vet Res 59:277–279

  9. Foster GW, Kinsella JM, Sheppard BJ, Cunningham MW (2009) Transmammary infection of free-ranging Florida panther neonates by Alaria marcianae (Trematoda: Diplostomatidae). J Parasitol 95:238–239

  10. Hartmann K (2012) Feline Leukemia Virus infection. In: Greene CE (ed) Infectious diseases of the dog and cat, 4th edn. Elsevier, St. Louis, p 126

  11. Jacobs DE, Arakawa A, Courtney CH, Gemmell MA, McCall JW, Myers GH, Vanparijs O (1994) World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) guidelines for evaluating the efficacy of anthelmintics for dogs and cats. Vet Parasitol 52:179–202

  12. Kramer F, Hammerstein R, Stoye M, Epe C (2006) Investigations into the prevention of prenatal and lactogenic Toxocara canis infections in puppies by application of moxidectin to the pregnant dog. J Vet Med B 53:218–223

  13. Kramer F, Epe C, Mencke N (2009) Investigations into the prevention of neonatal Ancylostoma caninum infections in puppies by application of imidacloprid 10 % plus moxidectin 2.5 % topical solution to the pregnant dog. Zoonoses Public Health 56:34–40

  14. Linde-Forsberg C, Eneroth A (1998) Parturition. In: Simson G (ed) Manual of small animal reproduction and neonatology, 1st edn. British Small Animal Veterinary Association, Shurdington, Cheltenham, pp 126–142

  15. Ludwig KG, Craig TM, Bowen JM, Ansari MM, Ley WB (1983) Efficacy of ivermectin in controlling Strongyloides westeri infections in foals. Am J Vet Res 44:314–316

  16. Lyons ET, Drudge JH, Tolliver SC (1973) On the life cycle of Strongyloides westeri in the equine. J Parasitol 59:780–787

  17. Miller GC (1981) Helminths and the transmammary route of infection. Parasitology 82:335–342

  18. Nwaorgu OC, Onyali IO (1990) Strongyloides papillosus: prenatal and transmammary infection in ewes. Rev Elev Med Vet Pays Trop 43:503–504

  19. Olsen OW, Lyons ET (1965) Life cycle of Uncinaria lucasi Stiles, 1901 (Nematoda: Ancylostomatidae) of fur seals, Callorhinus ursinus Linn., on the Pribilof Islands, Alaska. J Parasitol 51:689–700

  20. Roberts JA, Fernando ST, Sivanathan S (1990) Toxocara vitulorum in the milk of buffalo (Bubalus bubalis) cows. Res Vet Sci 49:289–291

  21. Shoop WL (1991) Vertical transmission of helminths: Hypobiosis and amphiparatenesis. Parasitol Today 7:51–54

  22. Shoop WL, Corkum KC (1983) Transmammary infection of paratenic and definitive hosts with Alaria marcianae (trematoda) mesocercariae. J Parasitol 69:731–735

  23. Shoop WL, Corkum KC (1984) Transmammary infection of newborn by larval trematodes. Science 223:1082–1083

  24. Sprent JFA (1956) The life history and development of Toxocara cati (Schrank 1788) in the domestic cat. Parasitology 46:54–78

  25. Stewart TB, Stone WM, Marti OG (1976) Strongyloides ransomi: prenatal and transmammary infection of pigs of sequential litters from dams experimentally exposed as weanlings. Am J Vet Res 37:541–544

  26. Swerczek TW, Nielsen SW, Helmboldt CF (1971) Transmammary passage of Toxocara cati in the cat. Am J Vet Res 32:89–92

  27. VICH Guideline 7: Efficacy of Anthelmintics: General Requirements. Veterinary International Cooperation on Harmonization, European Agency for the Evaluation of Medicinal Products, London, CVMP/VICH/832/99-corr, November 2000

  28. VICH Guideline 9: Good Clinical Practice. Veterinary International Cooperation on Harmonization, European Agency for the Evaluation of Medicinal Products, London, CVMP/VICH/595/98-Final, June 2000

  29. VICH Guideline 20: Efficacy of Anthelmintics: Specific Recommendations for Felines. Veterinary International Cooperation on Harmonization, European Agency for the Evaluation of Medicinal Products, London, CVMP/VICH/545/00-Final, June 2001

  30. Wilson PA, Cameron M, Scott DS (1978) Patterns of milk transmission of Strongyloides ratti. Parasitology 77:87–96

  31. Wetzel R (1951) Verbesserte McMaster-Kammer zum Auszahlen von Wurmeiern. Tieraeztli Umschau 6:209–210

  32. Wolken S, Schaper R, Mencke N, Kraemer F, Schnieder T (2009) Treatment and prevention of vertical transmission of Toxocara cati in cats with an emodepside/praziquantel spoton formulation. Parasitol Res 105(Suppl 1):S75–S81

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Correspondence to Claudia Böhm.

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Böhm, C., Petry, G., Schaper, R. et al. Prevention of Lactogenic Toxocara cati Infections in Kittens by Application of an Emodepside/Praziquantel Spot-on (Profender®) to the Pregnant Queen. Parasitol Res 114, 175–184 (2015). https://doi.org/10.1007/s00436-015-4523-y

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Keywords

  • Vertical Transmission
  • Late Pregnancy
  • Praziquantel
  • Worm Burden
  • Moxidectin