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Molecular characterization and expression of Rab7 from Clonorchis sinensis and its potential role in autophagy

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Abstract

Accumulating evidences suggest that Rab7 GTPase is important for the normal progression of autophagy. However, the role of Rab7 GTPase in regulation of autophagy in Clonorchis sinensis is not known. In this study, a gene encoding Rab7 was isolated from C. sinensis adult cDNA. Recombinant CsRab7 was expressed and purified from Escherichia coli. CsRab7 transcripts were detected in the cDNA of adult worm, metacercaria, cercaria, and egg of C. sinensis, and were highly expressed in the metacercaria. Immunohistochemical localization results revealed that CsRab7 was specifically deposited on the vitellarium and eggs of adult worm. Furthermore, EGFP signal of CsRab7WT and the active mutant CsRab7Q67L were associated with autophagic vesicles in transiently transfected 293T cells. It is concluded from the present study that CsRab7 GTPase possibly contributes to the development of C. sinensis and that the autophagy pathway could be an important site of action with respect to the developmental role of CsRab7 in C. sinensis.

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Acknowledgments

This work was supported by the National Key Basic Research and Development Project (973 project no. 2010CB530000), National Natural Science Foundation of China (no. 81101270 and no. 81171602), the National Important Sci-tech Special Projects (no. 2012ZX10004220), and the Fundamental Research Funds for the Central Universities (no. 3164015).

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Correspondence to Xuerong Li or Xinbing Yu.

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Supplementary Table 1

Specific primers used in the present study. (DOC 31 kb)

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Jia, F., Li, Y., Huang, Y. et al. Molecular characterization and expression of Rab7 from Clonorchis sinensis and its potential role in autophagy. Parasitol Res 112, 2461–2467 (2013). https://doi.org/10.1007/s00436-013-3409-0

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Keywords

  • Adult Worm
  • Autophagy Pathway
  • Clonorchiasis
  • Dimethyl Benzene
  • Autophagic Vesicle