Genetic differentiation between two synonymous echinostomes species, Artyfechinostomum malayanum and Artyfechinostomum sufrartyfex was determined by using the first and second internal transcribed spacers (ITS1 and ITS2), the non-coding region of rDNA as genetic makers. Of the 699 bp of combined ITS1 and ITS2 sequences examined, 18 variable nucleotide positions (2.58 %) were observed. Of these, 17 positions could be used as diagnostic position between these two sibling species, whereas the other one variation was intraspecific variation of A. malayanum. A clade of A. malayanum was closely aligned with A. sufrartyfex and clearly distance from the cluster of other echinostomes. Our results may sufficiently suggest that the current synonymy of these species is not valid.
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This research was supported by Thammasat University grant to C. Tantrawatpan and TRF-CHE-MSU (grant no. MRG5480009) to W. Saijuntha. We wish to acknowledge the Faculty of Medicine, Khon Kaen University, Overseas Visiting Professor Program grant. We would like to thanks Dr. Yukifumi Nawa, hosted by Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University for the comments and suggestions. We would like to thank the Deutsche Forschungsgemeinschaft (PE1611/1-3), the National Research Council of Thailand, the International Excellence Fund of ASEAN-EU Year of Science, Technology and Innovation, 2012 for providing funding for cooperative workshops.
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Tantrawatpan, C., Saijuntha, W., Sithithaworn, P. et al. Genetic differentiation of Artyfechinostomum malayanum and A. sufrartyfex (Trematoda: Echinostomatidae) based on internal transcribed spacer sequences. Parasitol Res 112, 437–441 (2013). https://doi.org/10.1007/s00436-012-3065-9
- Adult Worm
- Ventral Sucker
- ITS2 Sequence
- Internal Transcribe Space Sequence
- Rodent Host