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Classical and molecular cytogenetics of Khawia sinensis (Cestoda: Caryophyllidea), invasive parasite of carp, Cyprinus carpio

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Abstract

Chromosomes of the invasive tapeworm Khawia sinensis (Caryophyllidea), the specific parasite of common carp, were analyzed by means of conventional Giemsa staining and using fluorescent DAPI and YOYO-1 dyes, silver staining, and fluorescent in situ hybridization (FISH) with 18S rDNA probe. The karyotype is composed of eight pairs of metacentric and telocentric chromosomes (2n = 16, n = 3m + 5t, TCL = 42.54 μm). Constitutive heterochromatin was located at pericentromeric regions of all pairs, except for the largest metacentric pair (no. 1), which possessed no DAPI-positive band. FISH with rDNA probe revealed that both homologues of chromosome pair no. 6 carry a cluster of ribosomal arrays, which were located interstitially close to the centromere. Present results are compared with previous cytogenetic data on Khawia spp., and comments are made on the karyotypes with respect to their phylogenetic links.

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Acknowledgments

This work was supported by a project of the Slovak Research and Development Agency (no. LPP 0171–09), grants of the Grant Agency of the Slovak Republic (VEGA 2/0148/09, 2/0014/10 and 2/0129/12), and the Grant Agency of the Czech Republic (no. 524/08/0885), from the research projects of the Institute of Parasitology BC ASCR (Z60220578, LC 522) and the project Centre of Excellence for Parasitology (Code ITMS: 26220120022) based on the support of the Operational Programme “Research & Development” funded from the European Fund of Regional Development (rate 0.1).

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Correspondence to Martina Orosová.

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Orosová, M., Oros, M. Classical and molecular cytogenetics of Khawia sinensis (Cestoda: Caryophyllidea), invasive parasite of carp, Cyprinus carpio . Parasitol Res 110, 1937–1944 (2012). https://doi.org/10.1007/s00436-011-2720-x

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Keywords

  • Common Carp
  • Chromosome Pair
  • Pericentromeric Region
  • rDNA Site
  • Secondary Constriction