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Efficacy of Emodepside plus Toltrazuril Suspension (Procox® Oral Suspension for Dogs) against Prepatent and Patent Infection with Isospora canis and Isospora ohioensis-Complex in Dogs

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Three randomised, blinded and placebo-controlled laboratory studies were conducted to evaluate the efficacy of emodepside plus toltrazuril suspension (Procox® suspension for dogs) against Isospora canis and Isospora ohioensis-complex. Unweaned puppies were experimentally infected with sporulated oocysts of I. canis and/or I. ohioensis-complex. In each study, one group was treated during prepatency (2 or 4 days post infection) while dogs in the second group were treated individually after the onset of oocyst excretion of the respective coccidia species. The dogs were treated with the minimum therapeutic dose of 0.45 mg emodepside and 9 mg toltrazuril per kg body weight. Daily faecal oocyst counts from both groups were compared to placebotreated control groups to determine efficacy.

Dogs treated during prepatent I. canis or I. ohioensis-complex infection showed significantly lower oocyst counts for up to 12 days compared to the control group. Oocyst counts were reduced by 90.2 – 100 % while the control groups continued to exhibit an adequate infection, except for one study where efficacy against prepatent I. canis infection faded 13 days after treatment. Following treatment of patent I. canis or I. ohioensis-complex infections, significantly lowered oocyst counts were observed for up to 9 days compared to the control group. Faecal oocyst counts were reduced by 91.5 – 100 %. In all three studies the number of days with diarrhoea was significantly lower when dogs were treated during prepatent Isospora spp. infection compared to the control groups. No adverse drug reactions were observed during the studies. In conclusion, the studies demonstrated that emodepside plus toltrazuril suspension is an efficient coccidiocide for dogs.


  1. Altreuther G, Gasda N, Adler K, Hellmann K, Thurieau H, Schimmel A, Hutchens D, Krieger KJ (2011) Field evaluations of the efficacy and safety of emodepside plus toltrazuril (Procox® oral suspension for dogs) against naturally acquired nematode and Isospora spp. infections in dogs. Parasitol Res 109:21 – 28.

  2. Baek BK, Kim CS, Kim JH, Han KS, Kim YG (1993) Studies on isosporosis in dogs I: Isolation and sporulation of Isospora ohioensis. Korean J Parasitol 31:201 – 206.

  3. Barutzki D, Erber M, Boch J (1981) Möglichkeiten der Desinfektion bei Kokzidiose (Eimeria, Isospora, Toxoplasma, Sarcocystis). Berl Münch Tierärztl Wochenschr 94:451 – 454.

  4. Becker C, Heine J, Boch J (1981) Experimentelle Cystoisospora canis und C. ohioensis Infektionen beim Hund. Tierärztl Umschau 36: 336 – 341.

  5. Buehl IE, Prosl H, Mundt HC, Tichy AG, Joachim A (2006) Canine isosporosis – Epidemiology of field and experimental infections. J Vet Med B 53:482 – 487.

  6. Daugschies A, Mundt HC, Letkova V (2000) Toltrazuril treatment of cystisosporosis in dogs under experimental and field conditions. Parasitol Res 86:797 – 799.

  7. Dubey JP, Lindsay DS, Lappin MR (2009) Toxoplasmosis and other intestinal coccidial infections in cats and dogs. Vet Clin Small Anim 39:1009 – 1034.

  8. ESCCAP guideline 01, 2nd edn (September 2010): Worm control in cats and dogs. http://www.esccap.org/index.php/fuseaction/download/lrn_file/esccap-endo-guideline-v2-final-30sep2010.pdf.

  9. Fisher M (2002) Endoparasites in the dog and cat: 2. Protozoa. In Practice 24:146 – 153.

  10. Haberkorn A, Stoltefuss J (1987) Studies on the activity spectrum of toltrazuril, a new anti-coccidial agent. Vet Med Rev 1:22 – 32.

  11. Harder A, Haberkorn A (1989) Possible mode of action of toltrazuril: studies on two Eimeria species and mammalian and Ascaris suum enzymes. Parasitol Res 76:8 – 12.

  12. Lappin MR (2010) Update on the diagnosis and management of Isospora spp. infections in dogs and cats. Top Companion Anim Med 25:133 – 135.

  13. Lindsay DS, Dubey JP, Blagburn BL (1997) Biology of Isospora spp. from humans, nonhuman primates, and domestic animals. Clin Microbiol Rev 10:19 – 34.

  14. Mitchell SM, Zajac AM, Charles S, Duncan RB, Lindsay DS (2007) Cystoisospora canis Nemeséri, 1959 (syn. Isospora canis), infections in dogs: clinical signs, pathogenesis, and reproducible clinical disease in Beagle dogs fed oocysts. J Parasitol 93:345 – 352.

  15. Schimmel A , Schroeder I, Altreuther G, Settje T, Charles S, Wolken S, Kok DJ, Ketzis J, Young D, Hutchens D, Krieger KJ (2011) Efficacy of emodepside plus toltrazuril (Procox® oral suspension for dogs) against Toxocara canis, Uncinaria stenocephala and Ancylostoma caninum in dogs. Parasitol Res 109:1 – 8.

  16. Rommel M, Zielasko B (1981) Untersuchungen über den Lebenszyklus von Isospora burrowsi (Trayser und Todd, 1978) aus dem Hund. Berl Münch Tierärztl Wochenschr 94:87 – 90.

  17. Tenter, Deplazes (2006) Protozoeninfektionen von Hund und Katze – Isosporose. In: Veterinärmedizinische Parasitologie, Parey Verlag, Stuttgart, pp 421 – 425.

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Correspondence to Gertraut Altreuther.

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Altreuther, G., Gasda, N., Schroeder, I. et al. Efficacy of Emodepside plus Toltrazuril Suspension (Procox® Oral Suspension for Dogs) against Prepatent and Patent Infection with Isospora canis and Isospora ohioensis-Complex in Dogs. Parasitol Res 109, 9–20 (2011). https://doi.org/10.1007/s00436-011-2398-0

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  • Canis
  • Coccidiosis
  • Toltrazuril
  • Emodepside
  • Sporulated Oocyst