Cryptosporidiosis is a common protozoan disease observed in a wide range of vertebrate hosts, including ruminants. Cattle can be a potential reservoir of Cryptosporidium spp., leading to environmental contamination with oocysts of zoonotic species. The molecular characterization of Cryptosporidium spp. isolated from cattle from the state of São Paulo, Brazil, was accomplished using nested polymerase chain reaction for amplification of fragments of the 18S rRNA gene and the glycoprotein GP60 gene, following sequencing of amplified fragments. Positivity for Cryptosporidium was found in 10.7% (21/196) of the samples. Four species of Cryptosporidium were identified: C. andersoni, C. bovis, C. parvum subtype IIaA15G2R1, and C. ryanae. To the best of our knowledge, this is the first report of infection by C. ryanae and C. parvum IIaA15G2R1 in cattle from Brazil.
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Bellinzoni RC, Blackhall J, Terzolo HR, Moreira AR, Auza N, Mattion N, Micheo GL, La Torre JL, Scodeller EA (1990) Microbiology of diarrhoea in young beef and dairy calves in Argentina. Rev Argent Microbiol 22:130–136
Del Coco VF, Córdoba MA, Basualdo JA (2008) Cryptosporidium infection in calves from a rural area of Buenos Aires, Argentina. Vet Parasitol 158:31–35
Díaz-Lee A, Mercado R, Onuoha EO, Ozaki LS, Muñoz P, Muñoz V, Martínez FJ, Fredes F (2010) Cryptosporidium parvum in diarrheic calves detected by microscopy and identified by immunochromatographic and molecular methods. Vet Parasitol. doi:10.1016/j.vetpar.2010.11.001
Fayer R (2010) Taxonomy and species delimitation in Cryptosporidium. Exp Parasitol 124:90–97
Fayer R, Santín M, Trout JM (2007) Prevalence of Cryptosporidium species and genotypes in mature dairy cattle on farms in eastern United States compared with younger cattle from the same locations. Vet Parasitol 145:260–266
Feitosa FLF, Shimamura GM, Roberto T, Meireles MV, Nunes CM, Ciarlini PC, Borges AS (2004) Prevalência de criptosporidiose em bezerros na região de Araçatuba, Estado de São Paulo, Brasil. Cienc Rural 34:189–193
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Huber F, Silva S, Bomfim TCB, Teixeira KRS, Bello AR (2007) Genotypic characterization and phylogenetic analysis of Cryptosporidium sp. from domestic animals in Brazil. Vet Parasitol 150:65–74
Kváč M, Kouba M, Vítovec J (2006) Age-related and housing-dependence of Cryptosporidium infection of calves from dairy and beef herds in South Bohemia, Czech Republic. Vet Parasitol 137:202–209
Meireles MV (2010) Cryptosporidium infection in Brazil: implications for veterinary medicine and public health. Rev Bras Parasitol Vet 19:197–204
Meireles MV, Soares RM, Bonello F, Gennari SM (2007) Natural infection with zoonotic subtype of Cryptosporidium parvum in Capybara (Hydrochoerus hydrochaeris) from Brazil. Vet Parasitol 147:166–170
Meloni BP, Thompson RCA (1996) Simplified methods for obtaining purified oocysts from mice and for growing Cryptosporidium parvum in vitro. J Parasitol 82:757–762
Neira-Otero P, Muñoz-Saldías N, Sanchez-Moreno M, Rosales-Lombardo MJ (2005) Molecular characterization of Cryptosporidium species and genotypes in Chile. Parasitol Res 97:63–67
Peng MM, Wilson ML, Holland RE, Meshnick SR, Lal AA, Xiao L (2003) Genetic diversity of Cryptosporidium sp. in cattle in Michigan: implications for understanding the transmission dynamics. Parasitol Res 90:175–180
Plutzer J, Karanis P (2009) Genetic polymorphism in Cryptosporidium species: an update. Vet Parasitol 165:187–199
Santín M, Trout JM, Xiao L, Zhoub L, Greiner E, Fayer R (2004) Prevalence and age-related variation of Cryptosporidium species and genotypes in dairy calves. Vet Parasitol 22:103–117
Santín M, Trout JM, Fayer R (2008) A longitudinal study of cryptosporidiosis in dairy cattle from birth to 2 years of age. Vet Parasitol 155:15–23
Sevá AP, Funada MR, Souza SO, Nava A, Richtzenhain LJ, Soares RM (2010) Occurrence and molecular characterization of Cryptosporidium spp. isolated from domestic animals in a rural area surrounding Atlantic dry forest fragments in Teodoro Sampaio municipality, State of São Paulo, Brazil. Rev Bras Parasitol Vet 19:249–253
Silva DC, Homem CG, Nakamura AA, Teixeira WFP, Perri SHV, Meireles MV (2010) Physical, epidemiological, and molecular evaluation of infection by Cryptosporidium galli in Passeriformes. Parasitol Res 107:271–277
Surumay-Vilchez Q, Alfaro C (2000) Cryptosporidium spp. in farms in the eastern region of Venezuela. Invest Clin 41:245–250
Thomaz A, Meireles MV, Soares RM, Pena HFJ, Gennari SM (2007) Molecular identification of Cryptosporidium spp. from fecal samples of felines, canines and bovines in the state of São Paulo, Brazil. Vet Parasitol 150:291–296
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality tools. Nucleic Acids Res 24:4876–4882
Xiao L, Feng Y (2008) Zoonotic cryptosporidiosis. FEMS Immunol Med Microbiol 52:309–323
Xiao L, Alderisio K, Limor J, Royer M, Lal AA (2000) Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Appl Environ Microbiol 66:5492–5498
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Meireles, M.V., de Oliveira, F.P., Teixeira, W.F.P. et al. Molecular characterization of Cryptosporidium spp. in dairy calves from the state of São Paulo, Brazil. Parasitol Res 109, 949–951 (2011). https://doi.org/10.1007/s00436-011-2336-1
- Cryptosporidium Species
- BioEdit Sequence Alignment Editor
- Dairy Calf
- Subtype Level