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Comparative karyological analysis of four diplozoid species (Monogenea, Diplozoidae), gill parasites of cyprinid fishes

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Abstract

The present study has revealed new data on chromosome complements of diplozoid parasites, namely Diplozoon paradoxum from freshwater bream Abramis brama, Paradiplozoon bliccae from white bream Blicca bjoerkna, Paradiplozoon sapae from white-eye bream Ballerus sapa, and Paradiplozoon nagibinae from zope Ballerus ballerus. Particularly, D. paradoxum is characterized by four pairs (2n = 8) of long (up to 22.1 μm) chromosomes: pairs 1, 2, and 3 are metacentric and pair 4 acrocentric. Karyotypes of three Paradiplozoon species are nearly identical in number and morphological classification of chromosomes, each comprising diploid number of 14 one-armed chromosomes of very similar length ranging up to 12.5 μm in P. bliccae, 9.2 μm in P. sapae, and 9.9 μm in P. nagibinae. All four species are similar in their total complement length, ranging from 64.4 to 50.4 μm. Interspecific differences were found in location of secondary constriction: it is situated on short arm of the 1st chromosome pair in D. paradoxum, on long arm of the 7th pair in P. bliccae and on long arm of the 4th pairs in P. sapae and P. nagibinae. Phylogenetic interrelationship within the diplozoids and hypothetic karyotype evolution is here discussed.

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Acknowledgments

We are please to thank M. Oros, Parasitological Institute SAS, Košice, Slovakia, for a help in collecting and for identification of fish material from Slovakia. This work was supported by the Czech Science Foundation, No. 524/07/1610 and No. 526/09/H025, Long-Term Research Plan MSM 0021622416, Ichthyoparasitology Research Centre of the Ministry of Education, Youth and Sports of the Czech Republic (LC 522), by the Grant Agency of the Slovak Republic (VEGA 2/0148/09), and the project LPP-0126-07 of the Slovak Research and Development Agency.

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Correspondence to Marta Špakulová.

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Košková, E., Špakulová, M., Koubková, B. et al. Comparative karyological analysis of four diplozoid species (Monogenea, Diplozoidae), gill parasites of cyprinid fishes. Parasitol Res 108, 935–941 (2011). https://doi.org/10.1007/s00436-010-2135-0

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Keywords

  • Chromosome Pair
  • Centric Fusion
  • Nucleolar Organizer Region
  • Acrocentric Chromosome
  • Secondary Constriction